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验证数据展示

  • WB analysis of human brain using 13617-1-AP

    WB analysis of human brain using 13617-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13617-1-AP (RASGEF1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 13617-1-AP

    IHC staining of human stomach using 13617-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 13617-1-AP (RASGEF1B antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human stomach using 13617-1-AP

    IHC staining of human stomach using 13617-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 13617-1-AP (RASGEF1B antibody) at dilution of 1:50 (under 40x lens).

RASGEF1B Polyclonal antibody

RASGEF1B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13617-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

GPI gamma 4, GPIG4, RASGEF1B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman brain tissue, mouse lung tissue, mouse ovary tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13617-1-AP targets RASGEF1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4525

Product name: Recombinant human RASGEF1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-210 aa of BC036784

Sequence: MPQTPPFSAMFDSSGYNRNLYQSAEDSCGGLYYHDNNLLSGSLEALIQHLVPNVDYYPDRTYIFTFLLSSRLFMHPYELMAKVCHLCVEHQRLSDPDSDKNQMRKIAPKILQLLTEWTETFPYDFRDERMMRNLKDLAHRIASGEEVGNLNLARLLEFPGRAWVGNGAELCILISTASSLFCLWASPPHHILVYLVCQVRMIIPSHFKGI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RasGEF domain family, member 1B
别名 GPI gamma 4, GPIG4, RASGEF1B
计算分子量 55 kDa
观测分子量 50 kDa
GenBank蛋白编号BC036784
基因名称 RASGEF1B
Gene ID (NCBI) 153020
RRIDAB_10644147
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ0VAM2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RASGEF1B is a guanine-nucleotide exchange factor, whose expression is induced inmacrophages on stimulation by TLR2 and TLR4 agonists. It interacts with Ras family proteins. It also interacts with CCDC124 during cytokinesis.

实验方案

Product Specific Protocols
IHC protocol for RASGEF1B antibody 13617-1-APDownload protocol
WB protocol for RASGEF1B antibody 13617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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