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  • Featured Product
  • KD/KO Validated

RASEF Polyclonal antibody

RASEF Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA and More (2)

Conjugate

Unconjugated

Cat No : 11569-1-AP

Print datasheet

Synonyms

RAB45, Ras related protein Rab 45, RASEF

验证数据展示

  • WB analysis of mouse testis using 11569-1-AP

    WB analysis of mouse testis using 11569-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11569-1-AP (RASEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 11569-1-AP

    IHC staining of human pancreas cancer using 11569-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11569-1-AP (RASEF antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with RASEF antibody included

经过测试的应用

Positive WB detected inmouse testis tissue, mouse heart tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RASEF Ready-To-Use IHC Kit. RASEF primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11569-1-AP targets RASEF in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen RASEF fusion protein Ag2149 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAS and EF-hand domain containing
Synonyms RAB45, Ras related protein Rab 45, RASEF
Calculated Molecular Weight 740 aa, 83 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC023566
Gene Symbol RASEF
Gene ID (NCBI) 158158
RRIDAB_2238112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZ41
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RASEF antibody 11569-1-APDownload protocol
IHC protocol for RASEF antibody 11569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF,IP

Mol Cancer Res

RASEF is a novel diagnostic biomarker and a therapeutic target for lung cancer.

Authors - Oshita Hideto H
  • KD Validated
View Article