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验证数据展示

  • WB analysis of mouse testis using 11569-1-AP

    WB analysis of mouse testis using 11569-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11569-1-AP (RASEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 11569-1-AP

    IHC staining of mouse brain using 11569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11569-1-AP (RASEF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RASEF antibody included
  • IHC staining of mouse brain using 11569-1-AP

    IHC staining of mouse brain using 11569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11569-1-AP (RASEF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RASEF antibody included
  • Featured Product
  • KD/KO验证

RASEF Polyclonal antibody

RASEF Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11569-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (2)

别名

Ras-related protein Rab-45, Ras related protein Rab 45, Ras and EF-hand domain-containing protein, RAB45

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, mouse heart tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RASEF Ready-To-Use IHC Kit. RASEF primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11569-1-AP targets RASEF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse
文献引用反应性human
免疫原 RASEF fusion protein Ag2149 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAS and EF-hand domain containing
别名 Ras-related protein Rab-45, Ras related protein Rab 45, Ras and EF-hand domain-containing protein, RAB45
计算分子量 740 aa, 83 kDa
观测分子量 83 kDa
GenBank蛋白编号BC023566
基因名称 RASEF
Gene ID (NCBI) 158158
RRIDAB_2238112
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IZ41
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RASEF antibody 11569-1-APDownload protocol
IHC protocol for RASEF antibody 11569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF,IP

Mol Cancer Res

RASEF is a novel diagnostic biomarker and a therapeutic target for lung cancer.

Authors - Oshita Hideto H
  • KD Validated
View Article
humanIHC

Int J Clin Exp Pathol

High RASEF expression is associated with a significantly better prognosis in colorectal cancer

Authors - Xin Yu
View Article