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验证数据展示

  • WB analysis using 21164-1-AP

    WB analysis using 21164-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21164-1-AP (RASAL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 21164-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 21164-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21164-1-AP (RASAL3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RASAL3 Polyclonal antibody

RASAL3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21164-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

RAS protein activator like-3, RAS protein activator like 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, NK-92 cells, TF-1 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21164-1-AP targets RASAL3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 RASAL3 fusion protein Ag15189 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAS protein activator like 3
别名 RAS protein activator like-3, RAS protein activator like 3
计算分子量 1011 aa, 112 kDa
观测分子量112 kDa
GenBank蛋白编号BC030281
基因名称 RASAL3
Gene ID (NCBI) 64926
RRIDAB_3085641
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86YV0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RASAL3 belongs to the Ras GTPase-activating proteins (RasGAP) family and encodes a protein with pleckstrin homology (PH), C2, and Ras GTPase-activation protein (RasGAP) domains.

实验方案

Product Specific Protocols
WB protocol for RASAL3 antibody 21164-1-APDownload protocol
IHC protocol for RASAL3 antibody 21164-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols