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验证数据展示

  • IP experiment of Jurkat using 31586-1-AP (same clone as 31586-1-PBS)

    IP experiment of Jurkat using 31586-1-AP (same clone as 31586-1-PBS)

    IP result of anti-RASA2 (IP:31586-1-AP, 4ug; Detection:31586-1-AP 1:1000) with Jurkat cells lysate 1920 ug. This data was developed using the same antibody clone with 31586-1-PBS in a different storage buffer formulation.

  • IP experiment of A431 using 31586-1-AP (same clone as 31586-1-PBS)

    IP experiment of A431 using 31586-1-AP (same clone as 31586-1-PBS)

    IP result of anti-RASA2 (IP:31586-1-AP, 4ug; Detection:31586-1-AP 1:1000) with A431 cells lysate 1120 ug. This data was developed using the same antibody clone with 31586-1-PBS in a different storage buffer formulation.

  • IHC staining of human Hepatocellular carcinoma using 31586-1-AP (same clone as 31586-1-PBS)

    IHC staining of human Hepatocellular carcinoma using 31586-1-AP (same clone as 31586-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human Hepatocellular carcinoma slide using 31586-1-AP (RASA2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31586-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 31586-1-AP (same clone as 31586-1-PBS)

    IF Staining of A431 using 31586-1-AP (same clone as 31586-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RASA2 antibody (31586-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31586-1-PBS in a different storage buffer formulation.

RASA2 Polyclonal antibody, PBS Only

RASA2 Polyclonal Antibody for IHC, IF/ICC, IP, Indirect ELISA
Cat No. 31586-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, IP, Indirect ELISA

别名

GAP1M, RAS p21 protein activator 2, RASGAP

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31586-1-PBS targets RASA2 in IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35885

Product name: Recombinant human RASA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 700-850 aa of NM_001303245

Sequence: VLCRVSRCNQNRLSFYHPSVYLNGNWLCCQETGENTLGCKPCTAGVPADIQIDIDEDRETERIYSLFTLSLLKLQKMEEACGTIAVYQGPQKEPDDYSNFVIEDSVTTFKTIQQIKSIIEKLDEPHEKYRKKRSSSAKYGSKENPIVGKAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAS p21 protein activator 2
别名 GAP1M, RAS p21 protein activator 2, RASGAP
计算分子量96kDa
观测分子量 97 kDa
GenBank蛋白编号NM_001303245
基因名称 RASA2
Gene ID (NCBI) 5922
RRIDAB_3742452
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ15283
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ras GTPase-activating protein 2 (RASA2) is also named GAP1M and RASGAP. RASA2 was a critical RAS-specific GAP, inhibition of which permitted increased proliferation following TCR stimulation under conditions of immunosuppression (PMID: 37858490). RASA2 was identified as an inhibitor of RAS signaling in multiple human tumors, including melanoma, where inactivating RASA2 mutations have been found (PMID: 26502337). RASA2 is also one of the genes mutated in Noonan syndrome, a Rasopathy associated with developmental defects and elevated RAS activity (PMID: 25049390). RASA2 is expressed in both CD4+ and CD8+ T cells (PMID: 36002574).

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