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RASGAP Polyclonal antibody

RASGAP Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12935-1-AP

Print datasheet

Synonyms

CM AVM, CMAVM, GAP, GTPase activating protein, p120GAP, p120RASGAP, PKWS, Ras p21 protein activator, RASA, RASA1, RASGAP

验证数据展示

  • WB analysis using 12935-1-AP

    WB analysis using 12935-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12935-1-AP (RASGAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 12935-1-AP

    IP experiment of mouse testis using 12935-1-AP

    IP result of anti-RASGAP (IP:12935-1-AP, 4ug; Detection:12935-1-AP 1:4000) with mouse testis tissue lysate 1120 ug.

  • IHC staining of human testis using 12935-1-AP

    IHC staining of human testis using 12935-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 12935-1-AP

    IHC staining of human testis using 12935-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 12935-1-AP

    IF Staining of HeLa using 12935-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RASGAP antibody (12935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12935-1-AP targets RASGAP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen RASGAP fusion protein Ag3906 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAS p21 protein activator (GTPase activating protein) 1
Synonyms CM AVM, CMAVM, GAP, GTPase activating protein, p120GAP, p120RASGAP, PKWS, Ras p21 protein activator, RASA, RASA1, RASGAP
Calculated Molecular Weight 1047 aa, 116 kDa
Observed Molecular Weight 120 kDa, 95 kDa
GenBank Accession NumberBC033015
Gene Symbol RASA1
Gene ID (NCBI) 5921
RRIDAB_2175906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20936
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RASGAP antibody 12935-1-APDownload protocol
IHC protocol for RASGAP antibody 12935-1-APDownload protocol
IF protocol for RASGAP antibody 12935-1-APDownload protocol
IP protocol for RASGAP antibody 12935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Biochem Biophys Res Commun

miR-335 is involved in the rat epididymal development by targeting the mRNA of RASA1.

Authors - Wang Junfeng J
View Article
humanWB

Int J Clin Exp Pathol

Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration.

Authors - Xiaojuan Ye
View Article