验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, human placenta tissue, mouse placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16079-1-AP targets RARS2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | RARS2 fusion protein Ag9074 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | arginyl-tRNA synthetase 2, mitochondrial |
| 别名 | Arginine tRNA ligase, Arginyl tRNA synthetase like, ArgRS, DALRD2, PCH6, PRO1992, RARS2, RARSL |
| 计算分子量 | 578 aa, 66 kDa |
| 观测分子量 | 66-70 kDa |
| GenBank蛋白编号 | BC010420 |
| 基因名称 | RARS2 |
| Gene ID (NCBI) | 57038 |
| RRID | AB_3669230 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5T160 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RARS2 encodes the mitochondrial arginyl transfer RNA (tRNA) synthetase, an enzyme which belongs to the group of mitochondrial aminoacyl tRNA synthetases. These nuclear encoded mitochondrial proteins play a key role in mitochondrial protein translation by catalyzing the attachment of amino acids to their cognate tRNA molecules . Variants in RARS2 gene are associated with pontocerebellar hypoplasia type 6 (PCH6; MIM 611523), a rare mitochondrial encephalopathy with phenotype characterized by early onset seizures, profound developmental delay, and progressive pontocerebellar atrophy.(PMID: 27769281, PMID: 33209735)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RARS2 antibody 16079-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |