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EPAC2 Polyclonal antibody

EPAC2 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 19103-1-AP

Print datasheet

Synonyms

CAMP GEFII, CGEF2, EPAC 2, EPAC2, Nbla00496, RAPGEF4

验证数据展示

  • WB analysis of mouse brain using 19103-1-AP

    WB analysis of mouse brain using 19103-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19103-1-AP (EPAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 19103-1-AP

    IP experiment of mouse brain using 19103-1-AP

    IP result of anti-EPAC2 (IP:19103-1-AP, 4ug; Detection:19103-1-AP 1:600) with mouse brain tissue lysate 2560ug.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue, HepG2 cells
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19103-1-AP targets EPAC2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB,ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen EPAC2 fusion protein Ag5186 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rap guanine nucleotide exchange factor (GEF) 4
Synonyms CAMP GEFII, CGEF2, EPAC 2, EPAC2, Nbla00496, RAPGEF4
Calculated Molecular Weight 116 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC024004
Gene Symbol RAPGEF4
Gene ID (NCBI) 11069
RRIDAB_10597393
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WZA2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EPAC2 antibody 19103-1-APDownload protocol
IP protocol for EPAC2 antibody 19103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

J Cell Mol Med

Differential changes in cyclic adenosine 3'-5' monophosphate (cAMP) effectors and major Ca2+ handling proteins during diabetic cardiomyopathy

Authors - Victoria Chaoul
View Article
ratWB,IF

J Diabetes Investig

Epac2 activation mediates glucagon-induced glucogenesis in primary rat hepatocytes

Authors - Yusuke Shiozaki-Takagi
View Article