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RAP2A Polyclonal antibody

RAP2A Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 13789-1-AP

Print datasheet

Synonyms

K REV, KREV, RAP2, RAP2A, Ras related protein Rap 2a, RbBP 30

验证数据展示

  • WB analysis of A431 using 13789-1-AP

    WB analysis of A431 using 13789-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 13789-1-AP

    IF Staining of MCF-7 using 13789-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 13789-1-AP (RAP2A antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA431 cells, mouse lung tissue, K-562 cells, mouse brain tissue, PC-3 cells, HeLa cells
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13789-1-AP targets RAP2A in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen RAP2A fusion protein Ag4808 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAP2A, member of RAS oncogene family
Synonyms K REV, KREV, RAP2, RAP2A, Ras related protein Rap 2a, RbBP 30
Calculated Molecular Weight 183 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC041333
Gene Symbol RAP2A
Gene ID (NCBI) 5911
RRIDAB_2177303
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10114
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAP2A antibody 13789-1-APDownload protocol
IF protocol for RAP2A antibody 13789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

miR-9 and miR-124 synergistically affect regulation of dendritic branching via the AKT/GSK3β pathway by targeting Rap2a.

Authors - Qian Xue
View Article
humanIHC, WB

Clin Exp Med

RAP2A promotes apoptosis resistance of hepatocellular carcinoma cells via the mTOR pathway.

Authors - Jing-Ru Yang
View Article