验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, mouse lung tissue, K-562 cells, mouse brain tissue, PC-3 cells, HeLa cells |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
13789-1-AP targets RAP2A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag4808 Product name: Recombinant human RAP2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-183 aa of BC041333 Sequence: MREYKVVVLGSGGVGKSALTVQFVTGTFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYEKVPVILVGNKVDLESEREVSSSEGRALAEEWGCPFMETSAKSKTMVDELFAEIVRQMNYAAQPDKDDPCCSACNIQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAP2A, member of RAS oncogene family |
| 别名 | RbBP 30, RbBP-30, Ras-related protein Rap-2a, Ras related protein Rap 2a, RAP2 |
| 计算分子量 | 183 aa, 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC041333 |
| 基因名称 | RAP2A |
| Gene ID (NCBI) | 5911 |
| RRID | AB_2177303 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P10114 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RAP2A antibody 13789-1-AP | Download protocol |
| WB protocol for RAP2A antibody 13789-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep miR-9 and miR-124 synergistically affect regulation of dendritic branching via the AKT/GSK3β pathway by targeting Rap2a. | ||
Clin Exp Med RAP2A promotes apoptosis resistance of hepatocellular carcinoma cells via the mTOR pathway. |
