验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, SH-SY5Y cells, K-562 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86011-2-RR targets RAP1GAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | RAP1GAP fusion protein Ag5576 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | RAP1 GTPase activating protein |
| 别名 | KIAA0474, RAP1 GTPase activating protein, Rap1 GTPase-activating protein 1, RAP1GA1, RAP1GAP1 |
| 计算分子量 | 73 kDa |
| 观测分子量 | 85-95 kDa |
| GenBank蛋白编号 | BC054490 |
| 基因名称 | RAP1GAP |
| Gene ID (NCBI) | 5909 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P47736 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rap1 GTPase-activating protein (Rap1GAP) is an important tumor suppressor. Rap1GAP was associated with the AMPK and NF-κB signaling pathways and that Rap1GAP inhibited AMPK/SIRT1 and activated the NF-κB signaling pathway in model animals. Rap1GAP was verified to regulate the AMPK SIRT1/NF-κB signaling pathway and exacerbate the damage to myocardial cells caused by ischemia and hypoxia (PMID: 38320624). Rap1GAP is involved in Ang II-induced cardiomyocyte hypertrophy through the regulation of autophagy (PMID: 33936386). Rap1GAP, which is the GTPase-activating protein of Rap1, can bind to Rap1, promote the transformation of GTP from its active to inactive form, and terminate signal transduction (PMID: 21295042).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAP1GAP antibody 86011-2-RR | Download protocol |
| IHC protocol for RAP1GAP antibody 86011-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |