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验证数据展示

  • WB analysis using 88443-1-RR

    WB analysis using 88443-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88443-1-RR (RAP1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 88443-1-RR

    IF Staining of A549 using 88443-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using RAP1B antibody (88443-1-RR, Clone: 261087A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RAP1B Recombinant monoclonal antibody

RAP1B Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 88443-1-RR

产品说明书

CloneNo. 261087A12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

EC:3.6.5.2, GTP binding protein smg p21B, GTP-binding protein smg p21B, K REV, RAL1B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HUVEC cells, COS-7 cells, NIH/3T3 cells, C6 cells
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy RAP1B Ready-To-Use IHC Kit. RAP1B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

88443-1-RR targets RAP1B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1282

Product name: Recombinant human RAP1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-184 aa of BC000176

Sequence: MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDERVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLVRQINRKTPVPGKARKKSSCQLL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAP1B, member of RAS oncogene family
别名 EC:3.6.5.2, GTP binding protein smg p21B, GTP-binding protein smg p21B, K REV, RAL1B
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC000176
基因名称 RAP1B
Gene ID (NCBI) 5908
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP61224
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAP1B is a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. RAP1B localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. It also plays a role in regulating outside-in signaling in platelets.

实验方案

Product Specific Protocols
IF protocol for RAP1B antibody 88443-1-RRDownload protocol
WB protocol for RAP1B antibody 88443-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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