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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis of HeLa using 67146-1-Ig (same clone as 67146-1-PBS)

    WB analysis of HeLa using 67146-1-Ig (same clone as 67146-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • WB analysis using 67146-1-Ig (same clone as 67146-1-PBS)

    WB analysis using 67146-1-Ig (same clone as 67146-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67146-1-Ig (same clone as 67146-1-PBS)

    IF Staining of HepG2 using 67146-1-Ig (same clone as 67146-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67146-1-Ig (RANGAP1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

RanGAP1 Monoclonal antibody, PBS Only

RanGAP1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67146-1-PBS

产品说明书

CloneNo. 1F3A4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

1F3A4, KIAA1835, Ran GTPase-activating protein 1, SD

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67146-1-PBS targets RanGAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RanGAP1 fusion protein Ag26136 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Ran GTPase activating protein 1
别名 1F3A4, KIAA1835, Ran GTPase-activating protein 1, SD
计算分子量 64 kDa
观测分子量 64 kDa, 82 kDa
GenBank蛋白编号BC014044
基因名称 RANGAP1
Gene ID (NCBI) 5905
RRIDAB_2882445
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP46060
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RanGAP1 is a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. In mammalian cells, unmodified RanGAP1 is predominantly cytoplasmic, whereas modification by small ubiquitin-related modifier protein (SUMO) targets RanGAP1 to the cytoplasmic filaments of nuclear pore complex (NPC) where it forms a complex with RanBP2 and Ubc9. Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity. We got 64 kDa (cytoplasmic Ran GAP1) and 82 kDa (SUMO-1 modified Ran GAP1) in western blotting.