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验证数据展示

  • WB analysis using 82978-1-RR (same clone as 82978-1-PBS)

    WB analysis using 82978-1-RR (same clone as 82978-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82978-1-RR (RAN antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 82978-1-RR (same clone as 82978-1-PBS)

    WB analysis of HEK-293 using 82978-1-RR (same clone as 82978-1-PBS)

    WB result of RAN antibody (82978-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAN transfected HEK-293 cells. This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 82978-1-RR (same clone as 82978-1-PBS)

    FC experiment of A431 using 82978-1-RR (same clone as 82978-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human RAN (82978-1-RR, Clone:230317F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82978-1-RR (same clone as 82978-1-PBS)

    FC experiment of HeLa using 82978-1-RR (same clone as 82978-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human RAN (82978-1-RR, Clone:230317F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 82978-1-RR (same clone as 82978-1-PBS)

    IHC staining of human colon using 82978-1-RR (same clone as 82978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAN antibody included
  • IHC staining of rat kidney using 82978-1-RR (same clone as 82978-1-PBS)

    IHC staining of rat kidney using 82978-1-RR (same clone as 82978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAN antibody included
  • IHC staining of human placenta using 82978-1-RR (same clone as 82978-1-PBS)

    IHC staining of human placenta using 82978-1-RR (same clone as 82978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAN antibody included
  • IF Staining of HeLa using 82978-1-RR (same clone as 82978-1-PBS)

    IF Staining of HeLa using 82978-1-RR (same clone as 82978-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAN antibody (82978-1-RR, Clone: 230317F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 82978-1-RR (same clone as 82978-1-PBS)

    IHC staining of mouse kidney using 82978-1-RR (same clone as 82978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAN antibody included
  • Affinity and Kinetic Characterization of 82978-1-RR

    Affinity and Kinetic Characterization of 82978-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82978-1-RR against Human RAN were performed. The affinity constant is 3.08 nM.

  • Featured Product
  • KD/KO验证

RAN Recombinant monoclonal antibody, PBS Only

RAN Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82978-1-PBS

产品说明书

CloneNo. 230317F3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

ARA24, 230317F3, Androgen receptor-associated protein 24, EC:3.6.5.-, GTP binding nuclear protein Ran

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy RAN Ready-To-Use IHC Kit. RAN primary antibody included.

产品信息

82978-1-PBS targets RAN in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0748

Product name: Recombinant human RAN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-216 aa of BC004272

Sequence: MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAN, member RAS oncogene family
别名 ARA24, 230317F3, Androgen receptor-associated protein 24, EC:3.6.5.-, GTP binding nuclear protein Ran
计算分子量 25 kDa
观测分子量 24 kDa
GenBank蛋白编号BC004272
基因名称 RAN
Gene ID (NCBI) 5901
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62826
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAN is also named as ARA24, Ras-like protein TC4 and GTPase Ran, and belongs to the small GTPase superfamily family and the Ran family. GTPase Ran switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:32528950). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603, PMID:32528950).

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