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验证数据展示

  • WB analysis using 10469-1-AP

    WB analysis using 10469-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 10469-1-AP

    WB analysis of HEK-293 using 10469-1-AP

    WB result of RAN antibody (10469-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAN transfected HEK-293 cells.

  • IHC staining of human breast cancer using 10469-1-AP

    IHC staining of human breast cancer using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAN antibody included
  • IHC staining of human breast cancer using 10469-1-AP

    IHC staining of human breast cancer using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAN antibody included
  • IP experiment of mouse kidney using 10469-1-AP

    IP experiment of mouse kidney using 10469-1-AP

    IP result of anti-RAN (IP:10469-1-AP, 4ug; Detection:10469-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

  • IHC staining of mouse colon using 10469-1-AP

    IHC staining of mouse colon using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAN antibody included
  • IHC staining of mouse testis using 10469-1-AP

    IHC staining of mouse testis using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAN antibody included
  • IHC staining of rat colon using 10469-1-AP

    IHC staining of rat colon using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAN antibody included
  • IHC staining of human placenta using 10469-1-AP

    IHC staining of human placenta using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAN antibody included
  • FC experiment of Jurkat using 10469-1-AP

    FC experiment of Jurkat using 10469-1-AP

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug RAN Polyclonal antibody (10469-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • FC experiment of HeLa using 10469-1-AP

    FC experiment of HeLa using 10469-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAN Polyclonal antibody (10469-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IHC staining of human colon using 10469-1-AP

    IHC staining of human colon using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded normal colon slide using 10469-1-AP (RAN antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAN antibody included
  • IF Staining of HEK-293 using 10469-1-AP

    IF Staining of HEK-293 using 10469-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RAN antibody (10469-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

RAN Polyclonal antibody

RAN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10469-1-AP

产品说明书

引用文献(19)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

ARA24, Androgen receptor-associated protein 24, EC:3.6.5.-, GTP binding nuclear protein Ran, GTPase Ran

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA375 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse kidney tissue, NIH/3T3 cells, rat kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman breast cancer tissue, human colon tissue, human placenta tissue, human testis tissue, mouse colon tissue, mouse testis tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHeLa cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy RAN Ready-To-Use IHC Kit. RAN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10469-1-AP targets RAN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, chicken, macrobrachium rosenbergii
免疫原

CatNo: Ag0748

Product name: Recombinant human RAN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-216 aa of BC004272

Sequence: MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAN, member RAS oncogene family
别名 ARA24, Androgen receptor-associated protein 24, EC:3.6.5.-, GTP binding nuclear protein Ran, GTPase Ran
计算分子量 25 kDa
观测分子量 24 kDa
GenBank蛋白编号BC004272
基因名称 RAN
Gene ID (NCBI) 5901
RRIDAB_2176484
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62826
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for RAN antibody 10469-1-APDownload protocol
IHC protocol for RAN antibody 10469-1-APDownload protocol
IP protocol for RAN antibody 10469-1-APDownload protocol
WB protocol for RAN antibody 10469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP,IF

Cell Death Dis

RSL1D1 promotes the progression of colorectal cancer through RAN-mediated autophagy suppression.

Authors - Xunhua Liu
  • KD Validated
View Article
rat,humanWB

Diabetologia

Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.

Authors - Mu Zhang
View Article
ratWB,IP

Cell Commun Signal

Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression.

Authors - Abiola Abdulrahman Ayanlaja
View Article
humanIHC

Oncotarget

Identification of nasopharyngeal carcinoma metastasis-related biomarkers by iTRAQ combined with 2D-LC-MS/MS.

Authors - Zhen Chen
View Article
humanWB

Int J Biol Sci

Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression.

Authors - Yancheng Zhong
  • KD Validated
View Article
humanIF

J Cell Sci

A nuclear export sequence promotes CRM1-dependent targeting of the nucleoporin Nup214 to the nuclear pore complex.

Authors - Mohamed Hamed
View Article
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