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验证数据展示

  • WB analysis using 10469-1-AP

    WB analysis using 10469-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 10469-1-AP

    WB analysis of HEK-293 using 10469-1-AP

    WB result of RAN antibody (10469-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAN transfected HEK-293 cells.

  • IHC staining of human breast cancer using 10469-1-AP

    IHC staining of human breast cancer using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAN antibody included
  • IHC staining of human breast cancer using 10469-1-AP

    IHC staining of human breast cancer using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAN antibody included
  • IP experiment of mouse kidney using 10469-1-AP

    IP experiment of mouse kidney using 10469-1-AP

    IP result of anti-RAN (IP:10469-1-AP, 4ug; Detection:10469-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

  • IHC staining of mouse colon using 10469-1-AP

    IHC staining of mouse colon using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAN antibody included
  • IHC staining of mouse testis using 10469-1-AP

    IHC staining of mouse testis using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAN antibody included
  • IHC staining of rat colon using 10469-1-AP

    IHC staining of rat colon using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAN antibody included
  • IHC staining of human placenta using 10469-1-AP

    IHC staining of human placenta using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAN antibody included
  • FC experiment of Jurkat using 10469-1-AP

    FC experiment of Jurkat using 10469-1-AP

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug RAN Polyclonal antibody (10469-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • FC experiment of HeLa using 10469-1-AP

    FC experiment of HeLa using 10469-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAN Polyclonal antibody (10469-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IHC staining of human colon using 10469-1-AP

    IHC staining of human colon using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded normal colon slide using 10469-1-AP (RAN antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAN antibody included
  • IF Staining of HEK-293 using 10469-1-AP

    IF Staining of HEK-293 using 10469-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RAN antibody (10469-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

RAN Polyclonal antibody

RAN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10469-1-AP

产品说明书

引用文献(36)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

ARA24, Androgen receptor-associated protein 24, EC:3.6.5.-, GTP binding nuclear protein Ran, GTPase Ran

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA375 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse kidney tissue, NIH/3T3 cells, rat kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman breast cancer tissue, human colon tissue, human placenta tissue, human testis tissue, mouse colon tissue, mouse testis tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHeLa cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy RAN Ready-To-Use IHC Kit. RAN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10469-1-AP targets RAN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, chicken, macrobrachium rosenbergii
免疫原

CatNo: Ag0748

Product name: Recombinant human RAN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-216 aa of BC004272

Sequence: MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAN, member RAS oncogene family
别名 ARA24, Androgen receptor-associated protein 24, EC:3.6.5.-, GTP binding nuclear protein Ran, GTPase Ran
计算分子量 25 kDa
观测分子量 24 kDa
GenBank蛋白编号BC004272
基因名称 RAN
Gene ID (NCBI) 5901
RRIDAB_2176484
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62826
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for RAN antibody 10469-1-APDownload protocol
IF protocol for RAN antibody 10469-1-APDownload protocol
IHC protocol for RAN antibody 10469-1-APDownload protocol
IP protocol for RAN antibody 10469-1-APDownload protocol
WB protocol for RAN antibody 10469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu
View Article
humanWB,IF

Nat Cell Biol

Nuclear proteasomes buffer cytoplasmic proteins during autophagy compromise

Authors - So Jung Park
View Article
humanWB

Nat Commun

Thermal proteome profiling reveals fructose-1,6-bisphosphate as a phosphate donor to activate phosphoglycerate mutase 1

Authors - Yanling Zhang
View Article
mouseWB,IF

J Adv Res

Tankyrase activity is essential for asymmetric division and chromosome segregation in oocyte meiosis

Authors - Ping-Shuang Lu
View Article

Adv Sci (Weinh)

H3K36me3-Guided m6A Modification of Oncogenic L1CAM-AS1 Drives Macrophage Polarization and Immunotherapy Resistance in Hepatocellular Carcinoma

Authors - Teng Wang
View Article
mouseWB

Adv Sci (Weinh)

Arf1 GTPase Regulates Golgi-Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis

Authors - Kun-Huan Zhang
View Article
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