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验证数据展示

  • WB analysis using 88020-3-RR (same clone as 88020-3-PBS)

    WB analysis using 88020-3-RR (same clone as 88020-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88020-3-RR (RAMP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88020-3-PBS in a different storage buffer formulation.

  • WB analysis of mouse lung using 88020-3-RR (same clone as 88020-3-PBS)

    WB analysis of mouse lung using 88020-3-RR (same clone as 88020-3-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 88020-3-RR (RAMP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88020-3-PBS in a different storage buffer formulation.

  • WB analysis of rat lung using 88020-3-RR (same clone as 88020-3-PBS)

    WB analysis of rat lung using 88020-3-RR (same clone as 88020-3-PBS)

    rat lung tissue were subjected to SDS PAGE followed by western blot with 88020-3-RR (RAMP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88020-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 88020-3-RR (same clone as 88020-3-PBS)

    IHC staining of mouse lung using 88020-3-RR (same clone as 88020-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 88020-3-RR (RAMP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88020-3-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03316-1

    Cytometric bead array sample test of MP03316-1

    The mean RAMP2 concentration was determined to be 2.1 ng/mL in mouse brain tissue extract based on a 1.0 mg/mL extract load, 408.1 ng/mL in mouse lung tissue extract based on a 1.0 mg/mL extract load, 1.1 ng/mL in mouse retina tissue extract based on a 1.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP03316-1

    Cytometric bead array standard curve of MP03316-1

    Cytometric bead array standard curve of MP03316-1, MOUSE RAMP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88020-1-PBS. Detection antibody: 88020-3-PBS. Standard: Eg9065. Range: 0.156-20 ng/mL.

Mouse RAMP2 Recombinant monoclonal antibody, PBS Only (Detector)

RAMP2 Uni-rAb® Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 88020-3-PBS

产品说明书

CloneNo. 260084E10

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

Receptor activity-modifying protein 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

88020-3-PBS targets RAMP2 as part of a matched antibody pair:

MP03316-1: 88020-1-PBS capture and 88020-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg9065

Product name: Recombinant Mouse RAMP2 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 45-157 aa of NM_019444.2

Sequence: SPESLNQSLPESQNQSHPTEDSLVSKGKMEDYETHVLPCWYEYKSCMDSVKDWCNWTLISRHYSDLQNCLEYNADKFGLGFPNPLAENIILEAHLIHFANCSLVQPTFSDPPE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 receptor (calcitonin) activity modifying protein 2
别名 Receptor activity-modifying protein 2
计算分子量21 kDa
观测分子量20-35kDa
GenBank蛋白编号NM_019444.2
基因名称 Ramp2
Gene ID (NCBI) 54409
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9WUP0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAMP2 (receptor-activity-modifying protein) is a member of the RAMP family of single-transmembrane-domain proteins which consist of an N-terminal extracellular domain, a transmembrane region and a short intracellular C-terminal tail. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a G protein-coupled receptor, can function as either a calcitonin gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. RAMP1 transports the CRLR to the plasma membrane and then remains associated with it to function as a terminally glycosylated CGRP receptor, while RAMP2 and RAMP3 transfer the CRLR to the cell surface to generate receptors that are preferentially selective for adrenomedullin. (PMID: 9620797; 16188935)

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