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RALYL Polyclonal antibody

RALYL Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17179-1-AP

Print datasheet

Synonyms

hnRNP core protein C like 3, HNRPCL3, hRALYL, RALY RNA binding protein like, RALYL, RNA binding Raly like protein

验证数据展示

  • WB analysis of human brain using 17179-1-AP

    WB analysis of human brain using 17179-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17179-1-AP (RALYL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 17179-1-AP

    IHC staining of mouse brain using 17179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17179-1-AP (RALYL Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 17179-1-AP is RALYL Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen RALYL fusion protein Ag10898 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RALY RNA binding protein-like
Synonyms hnRNP core protein C like 3, HNRPCL3, hRALYL, RALY RNA binding protein like, RALYL, RNA binding Raly like protein
Calculated Molecular Weight 291 aa, 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC031090
Gene Symbol RALYL
Gene ID (NCBI) 138046
RRIDAB_2878355
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86SE5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RALYL antibody 17179-1-APDownload protocol
IHC protocol for RALYL antibody 17179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

Long noncoding RNA AFAP1-AS1 promotes tumor progression and invasion by regulating the miR-2110/Sp1 axis in triple-negative breast cancer.

Authors - Xiaohui Zhang
View Article