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验证数据展示

  • WB analysis of LNCaP using 68011-1-Ig (same clone as 68011-1-PBS)

    WB analysis of LNCaP using 68011-1-Ig (same clone as 68011-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68011-1-Ig (RALY antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 68011-1-Ig (same clone as 68011-1-PBS)

    IHC staining of human stomach cancer using 68011-1-Ig (same clone as 68011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68011-1-Ig (RALY antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RALY antibody included
  • IHC staining of human stomach cancer using 68011-1-Ig (same clone as 68011-1-PBS)

    IHC staining of human stomach cancer using 68011-1-Ig (same clone as 68011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68011-1-Ig (RALY antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RALY antibody included
  • IF Staining of A431 using 68011-1-Ig (same clone as 68011-1-PBS)

    IF Staining of A431 using 68011-1-Ig (same clone as 68011-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RALY antibody (68011-1-Ig, Clone: 2B10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 68011-1-Ig (same clone as 68011-1-PBS)

    FC experiment of A431 using 68011-1-Ig (same clone as 68011-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RALY (68011-1-Ig, Clone:2B10C3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

RALY Monoclonal antibody, PBS Only

RALY Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68011-1-PBS

产品说明书

CloneNo. 2B10C3

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

2B10C3, Autoantigen p542, Heterogeneous nuclear ribonucleoprotein C-like 2, hnRNP associated with lethal yellow protein homolog, hnRNP core protein C-like 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® RALY Ready-To-Use IHC Kit. RALY primary antibody included.

产品信息

68011-1-PBS targets RALY in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12250

Product name: Recombinant human RALY protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-307 aa of BC103753

Sequence: MSLKLQASNVTNKNDPKSINSRVFIGNLNTALVKKSDVETIFSKYGRVAGCSVHKGYAFVQYSNERHARAAVLGENGRVLAGQTLDINMAGEPKPDRPKGLKRAASAIYSGYIFDYDYYRDDFYDRLFDYRGRLSPVPVPRAVPVKRPRVTVPLVRRVKTNVPVKLFARSTAVTTSSAKIKLKSSELQAIKTELTQIKSNIDALLSRLEQIAAEQKANPDGKKKGDGGGASGGGGGGGGSGGGGSGGGGGGGSSRPPAPQENTTSEAGLPQGEARTRDDGDEEGLLTHSEEELEHSQDTDADDGALQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse))
别名 2B10C3, Autoantigen p542, Heterogeneous nuclear ribonucleoprotein C-like 2, hnRNP associated with lethal yellow protein homolog, hnRNP core protein C-like 2
计算分子量 307 aa, 33 kDa
观测分子量30-37 kDa
GenBank蛋白编号BC103753
基因名称 RALY
Gene ID (NCBI) 22913
RRIDAB_2918758
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9UKM9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNA-binding protein RALY is a protein that in humans is encoded by the RALY gene. In infectious mononucleosis, anti-EBNA-1 antibodies are produced which cross-react with multiple normal human proteins. The cross-reactivity is due to anti-gly/ala antibodies that cross-react with host proteins containing configurations like those in the EBNA-1 repeat. One such antigen is RALY which is a member of the heterogeneous nuclear ribonucleoprotein gene family. RALYhas two isoforms with the molecular weight of 30 kDa and 32 kDa, and it has many modification sites, which made it shows at 30-37 kDa by Western Blot.