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RALGAPB Polyclonal antibody

RALGAPB Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 28330-1-AP

Print datasheet

Synonyms

KIAA1219, p170, RALGAPB

验证数据展示

  • WB analysis using 28330-1-AP

    WB analysis using 28330-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28330-1-AP (RALGAPB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 28330-1-AP

    IHC staining of mouse cerebellum using 28330-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 28330-1-AP

    IHC staining of mouse cerebellum using 28330-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 28330-1-AP

    IHC staining of human liver cancer using 28330-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 28330-1-AP

    IHC staining of human liver cancer using 28330-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, rat brain
Positive IHC detected inhuman liver cancer tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28330-1-AP targets RALGAPB in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, rat, mouse
Immunogen RALGAPB fusion protein Ag28259 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA1219
Synonyms KIAA1219, p170, RALGAPB
Observed Molecular Weight160-170 kDa
GenBank Accession NumberBC047482
Gene Symbol KIAA1219
Gene ID (NCBI) 57148
RRIDAB_2918155
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86X10
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RALGAPB antibody 28330-1-APDownload protocol
IHC protocol for RALGAPB antibody 28330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols