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验证数据展示

  • WB analysis using 28330-1-AP

    WB analysis using 28330-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28330-1-AP (RALGAPB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 28330-1-AP

    IHC staining of mouse cerebellum using 28330-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 28330-1-AP

    IHC staining of mouse cerebellum using 28330-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 28330-1-AP

    IHC staining of human liver cancer using 28330-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 28330-1-AP

    IHC staining of human liver cancer using 28330-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RALGAPB Polyclonal antibody

RALGAPB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28330-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

Human, rat, mouse

应用

WB, IHC, ELISA

别名

KIAA1219, p170, RALGAPB

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, rat brain
Positive IHC detected inhuman liver cancer tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28330-1-AP targets RALGAPB in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, rat, mouse
文献引用反应性mouse
免疫原

CatNo: Ag28259

Product name: Recombinant human KIAA1219 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1222-1494 aa of BC047482

Sequence: NCCDDGEGSQQEVISSEDIGASIFNGQKKVLYYADALTEIAFVVPSPVESLTDSLESNISDQDSDSNMDLMPGILKQPSLTLELFPNHTDNLNSSQRLSPSSRMRKLPQGRPVPPLGPETRVSVVWVERYDDIENFPLSELMTEISTGVETTANSSTSLRSTTLEKEVPVIFIHPLNTGLFRIKIQGATGKFNMVIPLVDGMIVSRRALGFLVRQTVINICRRKRLESDSYSPPHVRRKQKITDIVNKYRNKQLEPEFYTSLFQEVGLKNCSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA1219
别名 KIAA1219, p170, RALGAPB
观测分子量160-170 kDa
GenBank蛋白编号BC047482
基因名称 RALGAPB
Gene ID (NCBI) 57148
RRIDAB_2918155
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86X10
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for RALGAPB antibody 28330-1-APDownload protocol
WB protocol for RALGAPB antibody 28330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Life Sci Alliance

RalGAP complexes control secretion and primary cilia in pancreatic disease

Authors - Lisa H Apken
View Article

Nat Commun

Structure and mechanism of the RalGAP tumor suppressor complex

Authors - René Rasche
View Article
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