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验证数据展示

  • WB analysis using 28330-1-AP

    WB analysis using 28330-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28330-1-AP (RALGAPB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 28330-1-AP

    IHC staining of mouse cerebellum using 28330-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 28330-1-AP

    IHC staining of mouse cerebellum using 28330-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 28330-1-AP

    IHC staining of human liver cancer using 28330-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 28330-1-AP

    IHC staining of human liver cancer using 28330-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RALGAPB Polyclonal antibody

RALGAPB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28330-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

Human, rat, mouse and More (1)

应用

WB, IHC, ELISA

别名

KIAA1219, p170, RALGAPB

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, rat brain
Positive IHC detected inhuman liver cancer tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28330-1-AP targets RALGAPB in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 Human, rat, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag28259

Product name: Recombinant human KIAA1219 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1222-1494 aa of BC047482

Sequence: NCCDDGEGSQQEVISSEDIGASIFNGQKKVLYYADALTEIAFVVPSPVESLTDSLESNISDQDSDSNMDLMPGILKQPSLTLELFPNHTDNLNSSQRLSPSSRMRKLPQGRPVPPLGPETRVSVVWVERYDDIENFPLSELMTEISTGVETTANSSTSLRSTTLEKEVPVIFIHPLNTGLFRIKIQGATGKFNMVIPLVDGMIVSRRALGFLVRQTVINICRRKRLESDSYSPPHVRRKQKITDIVNKYRNKQLEPEFYTSLFQEVGLKNCSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA1219
别名 KIAA1219, p170, RALGAPB
观测分子量160-170 kDa
GenBank蛋白编号BC047482
基因名称 RALGAPB
Gene ID (NCBI) 57148
RRIDAB_2918155
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86X10
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for RALGAPB antibody 28330-1-APDownload protocol
WB protocol for RALGAPB antibody 28330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Life Sci Alliance

RalGAP complexes control secretion and primary cilia in pancreatic disease

Authors - Lisa H Apken
View Article

Nat Commun

Structure and mechanism of the RalGAP tumor suppressor complex

Authors - René Rasche
View Article

Nature

Dietary cholesterol activates a Ral-dependent pathway driving LDLR turnover

Authors - Xue Feng
View Article
humanIHC

Biochem Biophys Res Commun

Circ-ZBTB38 as an oncogenic circular RNA: Mechanisms in disrupting RalGAP complexes and its clinical value in melanoma.

Authors - Wanqi Zhang
View Article
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