验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, human brain tissue, rat brain tissue, fetal human brain tissue, MCF-7 cells, HeLa cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 5 publications below |
IHC | See 2 publications below |
产品信息
13629-1-AP targets RALA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | RALA fusion protein Ag4547 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | v-ral simian leukemia viral oncogene homolog A (ras related) |
别名 | RAL, RALA, Ras related protein Ral A |
计算分子量 | 206 aa, 24 kDa |
观测分子量 | 24 kDa |
GenBank蛋白编号 | BC039858 |
基因名称 | RALA |
Gene ID (NCBI) | 5898 |
RRID | AB_2269251 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P11233 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RALA belongs to the small GTPase superfamily. Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. RALA plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. As an effector of the proto-oncogene Ras, it play an important role in the molecular pathology of human malignancies.
实验方案
Product Specific Protocols | |
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WB protocol for RALA antibody 13629-1-AP | Download protocol |
IHC protocol for RALA antibody 13629-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Metab Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans. | ||
J Extracell Vesicles A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells. | ||
Front Chem Design, Synthesis, and Biological Evaluation of Pyrano[2,3-c]-pyrazole-Based RalA Inhibitors Against Hepatocellular Carcinoma. | ||
Hum Pathol Papillary renal neoplasm with reverse polarity: a clinicopathologic study of 43 cases with a focus on the expression of KRAS signaling pathway downstream effectors | ||
Intractable Rare Dis Res A comparative proteomics study on matrix vesicles of osteoblast-like Saos-2 and U2-OS cells. | ||