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RALA Polyclonal antibody

RALA Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13629-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

RAL, RALA, Ras related protein Ral A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue, fetal human brain tissue, MCF-7 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RALA Ready-To-Use IHC Kit. RALA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13629-1-AP targets RALA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 RALA fusion protein Ag4547 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-ral simian leukemia viral oncogene homolog A (ras related)
别名 RAL, RALA, Ras related protein Ral A
计算分子量 206 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC039858
基因名称 RALA
Gene ID (NCBI) 5898
RRIDAB_2269251
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11233
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RALA belongs to the small GTPase superfamily. Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. RALA plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. As an effector of the proto-oncogene Ras, it play an important role in the molecular pathology of human malignancies.

实验方案

Product Specific Protocols
WB protocol for RALA antibody 13629-1-APDownload protocol
IHC protocol for RALA antibody 13629-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Authors - Xiao Wang
humanWB

J Extracell Vesicles

A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells.

Authors - Charles P Hinzman
humanWB, IHC

Front Chem

Design, Synthesis, and Biological Evaluation of Pyrano[2,3-c]-pyrazole-Based RalA Inhibitors Against Hepatocellular Carcinoma.

Authors - Yuting Wang
humanIHC

Hum Pathol

Papillary renal neoplasm with reverse polarity: a clinicopathologic study of 43 cases with a focus on the expression of KRAS signaling pathway downstream effectors

Authors - Bohyun Kim
humanWB

Intractable Rare Dis Res

A comparative proteomics study on matrix vesicles of osteoblast-like Saos-2 and U2-OS cells.

Authors - Liang Jiang
mouseWB

Oncotarget

Evaluation of cancer-derived myocardial impairments using a mouse model.

Authors - Yoshihiro Miyagawa
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