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Species Reactivity
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Species Reactivity
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验证数据展示

  • WB analysis using 28217-1-AP

    WB analysis using 28217-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28217-1-AP (RAI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of SW480 using 28217-1-AP

    IF Staining of SW480 using 28217-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SW480 cells using RAI2 antibody (28217-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

RAI2 Polyclonal antibody

RAI2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28217-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

retinoic acid induced 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, IMR-32 cells, SK-N-SH cells, TT cells
Positive IF/ICC detected inSW480 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28217-1-AP targets RAI2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 RAI2 fusion protein Ag28253 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 retinoic acid induced 2
别名 retinoic acid induced 2
计算分子量 57 kDa
观测分子量70 kDa
GenBank蛋白编号BC027937
基因名称 RAI2
Gene ID (NCBI) 10742
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5P3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Retinoic acid-induced 2 (RAI2) was initially described as a retinoic acid-inducible gene, which has been assumed to be involved in development. RAI2 was initially related to cell differentiation and induced by retinoic acid and has been identified as an emerging tumor suppressor in breast cancer and colorectal cancer. RAI2 is frequently methylated in human CRC, and the expression of RAI2 is regulated by promoter region methylation (PMID: 29796120).

实验方案

Product Specific Protocols
WB protocol for RAI2 antibody 28217-1-APDownload protocol
IF protocol for RAI2 antibody 28217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols