验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
17084-1-AP targets RAI1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag9046 Product name: Recombinant human RAI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC021209 Sequence: MQSFRERCGFHGKQQNYQQTSQETSRLENYRQPSQAGLSCDRQRLLAKDYYNPQPYPSYEGGAGTPSGTAAAVAADKYHRGSKALPTQQGLQGRPAFPGYGVQDSSPYPGRYAGEESLQAWGAPQPPPPQPQPLPAGVAKYDENLMKKTAVPPSRQYAEQGAQVPFRTHSLHVQQPPPPQQPLAYPKLQRQKLQNDIASPLPFPQGTHFPQHSQSFPTSSTYSSSVQGGGQGAHSYKSCTAPTAQPHDRPLTASSSLAPGQRVQNLHAYQSGRLSYDQQQQQQQQQQQQQQALQSRHHAQETLHYQNLAKYQHYGQQGQGYCQPDAAVRTPEQYYQTFSPSSSHSPARSV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | retinoic acid induced 1 |
| 别名 | KIAA1820, retinoic acid induced 1, Retinoic acid-induced protein 1, SMCR, SMS |
| 计算分子量 | 1906aa,203 kDa; 966aa,104 kDa |
| 观测分子量 | 250-260 kDa |
| GenBank蛋白编号 | BC021209 |
| 基因名称 | RAI1 |
| Gene ID (NCBI) | 10743 |
| RRID | AB_2284824 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z5J4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The RAI1 (Retinoic Acid Induced 1) gene is located on the short arm of chromosome 17. The RAI1 protein it encodes is a crucial transcriptional regulator, playing a central role in processes such as neurodevelopment, sleep regulation, circadian rhythms, and energy metabolism. This protein contains a chromatin domain that binds to specific DNA sequences and recruits other transcriptional co-factors to activate or repress the expression of downstream target genes. The RAI1 gene is extremely sensitive to haploinsufficiency, and its dysfunction is closely linked to several severe neurodevelopmental disorders. For instance, RAI1 haploinsufficiency causes Smith-Magenis syndrome, while duplication of the RAI1 gene is associated with Potocki-Lupski syndrome. Consequently, the RAI1 protein is a key molecule for understanding brain function and behavioral regulation, and a major focus of research for related genetic syndromes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAI1 antibody 17084-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
