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验证数据展示

  • WB analysis of HeLa using 23926-1-AP

    WB analysis of HeLa using 23926-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23926-1-AP (MOK antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 23926-1-AP

    FC experiment of HeLa using 23926-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MOK Polyclonal antibody (23926-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human hepatocirrhosis using 23926-1-AP

    IHC staining of human hepatocirrhosis using 23926-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human hepatocirrhosis using 23926-1-AP

    IHC staining of human hepatocirrhosis using 23926-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 23926-1-AP

    IF Staining of HeLa using 23926-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23926-1-AP (MOK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

MOK Polyclonal antibody

MOK Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 23926-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

RAGE, EC:2.7.11.22, MOK protein kinase, RAGE 1, RAGE1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman hepatocirrhosis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23926-1-AP targets MOK in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 MOK fusion protein Ag20849 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 renal tumor antigen
别名 RAGE, EC:2.7.11.22, MOK protein kinase, RAGE 1, RAGE1
计算分子量 231 aa, 27 kDa
观测分子量 42 kDa, 85 kDa
GenBank蛋白编号BC053536
基因名称 RAGE
Gene ID (NCBI) 5891
RRIDAB_2879364
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQ07
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MOK antibody 23926-1-APDownload protocol
IHC protocol for MOK antibody 23926-1-APDownload protocol
IF protocol for MOK antibody 23926-1-APDownload protocol
FC protocol for MOK antibody 23926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

Proteomics and phosphoproteomics of chordoma biopsies reveal alterations in multiple pathways and aberrant kinases activities

Authors - Jing Hang
  • KD Validated
View Article
WB

J Biol Chem

Serine/Threonine Kinase Unc-51-like Kinase-1 (Ulk1) Phosphorylates the Co-chaperone Cell Division Cycle Protein 37 (Cdc37) and Thereby Disrupts the Stability of Cdc37 Client Proteins.

Authors - Ran Li
View Article