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验证数据展示

  • WB analysis of HeLa using 23926-1-AP

    WB analysis of HeLa using 23926-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23926-1-AP (MOK antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 23926-1-AP

    FC experiment of HeLa using 23926-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MOK Polyclonal antibody (23926-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IHC staining of human hepatocirrhosis using 23926-1-AP

    IHC staining of human hepatocirrhosis using 23926-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human hepatocirrhosis using 23926-1-AP

    IHC staining of human hepatocirrhosis using 23926-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 23926-1-AP

    IF Staining of HeLa using 23926-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23926-1-AP (MOK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

MOK Polyclonal antibody

MOK Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 23926-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

RAGE, EC:2.7.11.22, MOK protein kinase, RAGE 1, RAGE1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman hepatocirrhosis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23926-1-AP targets MOK in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag20849

Product name: Recombinant human RAGE protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-231 aa of BC053536

Sequence: MKNYKAIGKIGEGTFSEVMKMQSLRDGNYYACKQMKQRFESIEQVNNLREIQALRRLNPHPNILMLHEVVFDRKSGSLALICELMDMNIYELIRGRRYPLSEKKIMHYMYQLCKSLDHIHRNGIFHRDVKPENILIKQDVLKLGDFGSCRSVYSKQPYTEYISTRWYRAPECLLTDGFYTYKMDLWSAGCVFYEIASLQPLFPGVNELDQISKIHDVIGTPAQKILTKFKQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 renal tumor antigen
别名 RAGE, EC:2.7.11.22, MOK protein kinase, RAGE 1, RAGE1
计算分子量 231 aa, 27 kDa
观测分子量 42 kDa, 85 kDa
GenBank蛋白编号BC053536
基因名称 RAGE
Gene ID (NCBI) 5891
RRIDAB_2879364
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQ07
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for MOK antibody 23926-1-APDownload protocol
IF protocol for MOK antibody 23926-1-APDownload protocol
IHC protocol for MOK antibody 23926-1-APDownload protocol
WB protocol for MOK antibody 23926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

J Biol Chem

Serine/Threonine Kinase Unc-51-like Kinase-1 (Ulk1) Phosphorylates the Co-chaperone Cell Division Cycle Protein 37 (Cdc37) and Thereby Disrupts the Stability of Cdc37 Client Proteins.

Authors - Ran Li
View Article
humanWB

Front Oncol

Proteomics and phosphoproteomics of chordoma biopsies reveal alterations in multiple pathways and aberrant kinases activities

Authors - Jing Hang
  • KD Validated
View Article
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