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验证数据展示

  • WB analysis of A375 using 11825-1-AP (same clone as 11825-1-PBS)

    WB analysis of A375 using 11825-1-AP (same clone as 11825-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 11825-1-AP (same clone as 11825-1-PBS)

    IHC staining of human lymphoma using 11825-1-AP (same clone as 11825-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11825-1-AP (RAG2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11825-1-AP (same clone as 11825-1-PBS)

    IF Staining of HeLa using 11825-1-AP (same clone as 11825-1-PBS)

    Immunofluorescent analysis of Hela cells, using RAG2 antibody 11825-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse thymus using 11825-1-AP (same clone as 11825-1-PBS)

    IP experiment of mouse thymus using 11825-1-AP (same clone as 11825-1-PBS)

    IP result of anti-RAG2 (IP:11825-1-AP, 4ug; Detection:11825-1-AP 1:500) with mouse thymus tissue lysate 1000 ug. This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

RAG2 Polyclonal antibody, PBS Only

RAG2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11825-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

RAG 2, RAG-2, V(D)J recombination-activating protein 2

缓冲液配方:  PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

11825-1-PBS targets RAG2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2393

Product name: Recombinant human RAG2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 175-527 aa of BC022397

Sequence: VADCLPCVFLVDFEFGCATSYILPELQDGLSFHVSIAKNDTIYILGGHSLANNIRPANLYRIRVDLPLGSPAVNCTVLPGGISVSSAILTQTNNDEFVIVGGYQLENQKRMICNIISLEDNKIEIREMETPDWTPDIKHSKIWFGSNTGNGTVFLGIPGDNKQVVSEGFYFYMLKCAEDDTNEEQTTFTNSQTSTEDPGDSTPFEDSEEFCFSAEANSFDGDDEFDTYNEDDEEDESETGYWITCCPTCDVDINTWVPFYSTELNKPAMIYCSHGDGHWVHAQCMDLAERTLIHLSAGSNKYYCNEHVEIARALHTPQRVLPLKKPPMKSLRKKGSGKILTPAKKSFLRRLFD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 recombination activating gene 2
别名 RAG 2, RAG-2, V(D)J recombination-activating protein 2
计算分子量 527 aa, 59 kDa
观测分子量 57-62 kDa
GenBank蛋白编号BC022397
基因名称 RAG2
Gene ID (NCBI) 5897
RRIDAB_2238062
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55895
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3

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