验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, DC2.4 cells, HEK-293 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66592-1-Ig targets RAF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag25423 Product name: Recombinant human RAF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-59 aa of BC018119 Sequence: MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIR 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | v-raf-1 murine leukemia viral oncogene homolog 1 |
| 别名 | Proto oncogene c RAF, NS5, EC:2.7.11.1, CRAF, c Raf |
| 计算分子量 | 648 aa, 73 kDa |
| 观测分子量 | 70-75 kDa |
| GenBank蛋白编号 | BC018119 |
| 基因名称 | RAF1 |
| Gene ID (NCBI) | 5894 |
| RRID | AB_2881952 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04049 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RAF1 antibody 66592-1-Ig | Download protocol |
| IHC protocol for RAF1 antibody 66592-1-Ig | Download protocol |
| WB protocol for RAF1 antibody 66592-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res Bufalin inhibits hepatocellular carcinoma progression by blocking EGFR-mediated RAS-RAF-MEK-ERK pathway activation | ||
Cell Mol Biol Lett Exosomal DNAJB11 promotes the development of pancreatic cancer by modulating the EGFR/MAPK pathway | ||
Cell Death Dis Oncogenic RTKs sensitize cancer cells to ferroptosis via c-Myc mediated upregulation of ACSL4
| ||
Int J Biol Sci Exosomal SLC16A1-AS1-induced M2 macrophages polarization facilitates hepatocellular carcinoma progression | ||
Mol Ther Pancreatic cancer-derived extracellular vesicles remodel the tumor microenvironment and enhance chemoresistance by delivering KRASG12D protein to cancer-associated fibroblasts | ||
NPJ Precis Oncol GLUD1 supports ovarian cancer progression by counteracting anoikis via ARAF/MEK/ERK signaling. |
