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RAE1 Polyclonal antibody

RAE1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20491-1-AP

Print datasheet

Synonyms

MIG14, Mnrp41, mRNA export factor, MRNP41, RAE1, Rae1 protein homolog

验证数据展示

  • WB analysis of BxPC-3 using 20491-1-AP

    WB analysis of BxPC-3 using 20491-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20491-1-AP (RAE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 20491-1-AP

    IHC staining of human testis using 20491-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20491-1-AP (RAE1 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HepG2 using 20491-1-AP

    IF Staining of HepG2 using 20491-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 20491-1-AP (RAE1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inBxPC-3 cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20491-1-AP is RAE1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen RAE1 fusion protein Ag12102 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAE1 RNA export 1 homolog (S. pombe)
Synonyms MIG14, Mnrp41, mRNA export factor, MRNP41, RAE1, Rae1 protein homolog
Calculated Molecular Weight 368 aa, 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC103754
Gene Symbol RAE1
Gene ID (NCBI) 8480
RRIDAB_2878693
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78406
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAE1 antibody 20491-1-APDownload protocol
IHC protocol for RAE1 antibody 20491-1-APDownload protocol
IF protocol for RAE1 antibody 20491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB，IHC

Cancer Sci

Mitotic checkpoint regulator RAE1 promotes tumor growth in colorectal cancer.

Authors - Yuta Kobayashi
View Article
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu
View Article

Dev Cell

Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination

Authors - Li Li
View Article