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验证数据展示

  • WB analysis using 87990-1-RR

    WB analysis using 87990-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87990-1-RR (RAD51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 87990-1-RR

    WB analysis using 87990-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87990-1-RR (RAD51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 87990-1-RR

    IP experiment of HeLa using 87990-1-RR

    IP result of anti-RAD51 (IP:87990-1-RR, 4ug; Detection:87990-1-RR 1:3000) with HeLa cells lysate 1000 ug.

  • Affinity of 87990-1-RR

    Affinity of 87990-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87990-1-RR against Human RAD51 were performed. The affinity constant is below 1 pM.

RAD51 Recombinant monoclonal antibody

RAD51 Uni-rAb® Recombinant Antibody for WB, IP, ELISA
Cat No. 87990-1-RR

产品说明书

CloneNo. 260095G11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

BRCC5, HRAD51, HsRad51, HsT16930, RAD51 homolog A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, 4T1 cells, NIH/3T3 cells, HSC-T6 cells, K-562 cells, MCF-7 cells, Jurkat cells
Positive IP detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAD51 Ready-To-Use IHC Kit. RAD51 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87990-1-RR targets RAD51 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6823

Product name: Recombinant human RAD51 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-242 aa of BC001459

Sequence: MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
别名 BRCC5, HRAD51, HsRad51, HsT16930, RAD51 homolog A
计算分子量 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC001459
基因名称 RAD51
Gene ID (NCBI) 5888
RRIDAB_3745846
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ06609
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAD51 plays a critical role in the maintenance of genomic integrity by functioning in the repair of DNA double-strand breaks. It mediates homologous pairing and strand exchange in recombinatory structures known as RAD51foci in the nucleus. It also can bind to single and double stranded DNA and exhibits DNA-dependent ATPase activity. In addition, it has a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

实验方案

Product Specific Protocols
IP protocol for RAD51 antibody 87990-1-RRDownload protocol
WB protocol for RAD51 antibody 87990-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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