验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, 4T1 cells, NIH/3T3 cells, K-562 cells, HSC-T6 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy RAD51 Ready-To-Use IHC Kit. RAD51 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 3 publications below |
产品信息
67024-1-Ig targets RAD51 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | RAD51 fusion protein Ag7410 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) |
| 别名 | 4A12B8, BRCC5, HRAD51, HsRad51, HsT16930 |
| 计算分子量 | 37 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC001459 |
| 基因名称 | RAD51 |
| Gene ID (NCBI) | 5888 |
| RRID | AB_2882339 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q06609 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAD51 plays a critical role in the maintenance of genomic integrity by functioning in the repair of DNA double-strand breaks. It mediates homologous pairing and strand exchange in recombinatory structures known as RAD51foci in the nucleus. It also can bind to single and double stranded DNA and exhibits DNA-dependent ATPase activity. In addition, it has a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAD51 antibody 67024-1-Ig | Download protocol |
| IHC protocol for RAD51 antibody 67024-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology Minimizing adverse effects of Cerenkov radiation induced photodynamic therapy with transformable photosensitizer-loaded nanovesicles. | ||
Int J Mol Sci Combined PARP and Dual Topoisomerase Inhibition Potentiates Genome Instability and Cell Death in Ovarian Cancer | ||
J Med Chem Rational Design of PARP1/c-Met Dual Inhibitors for Overcoming PARP1 Inhibitor Resistance Induced by c-Met Overexpression | ||
Cell Death Dis Iron promotes ovarian cancer malignancy and advances platinum resistance by enhancing DNA repair via FTH1/FTL/POLQ/RAD51 axis | ||
Mol Cancer NSUN6-mediated 5-methylcytosine modification of NDRG1 mRNA promotes radioresistance in cervical cancer | ||
Cell Death Dis Overexpression of miR-124 enhances the therapeutic benefit of TMZ treatment in the orthotopic GBM mice model by inhibition of DNA damage repair |
