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验证数据展示

  • WB analysis using 29390-1-AP (same clone as 29390-1-PBS)

    WB analysis using 29390-1-AP (same clone as 29390-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29390-1-AP (RAD50 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 29390-1-AP (same clone as 29390-1-PBS)

    WB analysis of MCF-7 using 29390-1-AP (same clone as 29390-1-PBS)

    WB result of RAD50 antibody (29390-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD50 transfected MCF-7 cells. This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 29390-1-AP (same clone as 29390-1-PBS)

    IHC staining of human breast cancer using 29390-1-AP (same clone as 29390-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAD50 antibody included
  • IHC staining of human breast cancer using 29390-1-AP (same clone as 29390-1-PBS)

    IHC staining of human breast cancer using 29390-1-AP (same clone as 29390-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAD50 antibody included
  • IF Staining of mouse testis using 29390-1-AP (same clone as 29390-1-PBS)

    IF Staining of mouse testis using 29390-1-AP (same clone as 29390-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using RAD50 antibody (29390-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 29390-1-AP (same clone as 29390-1-PBS)

    IF Staining of HeLa using 29390-1-AP (same clone as 29390-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAD50 antibody (29390-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 29390-1-AP (same clone as 29390-1-PBS)

    IF Staining of U2OS using 29390-1-AP (same clone as 29390-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RAD50 antibody (29390-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RAD50 Polyclonal antibody, PBS Only

RAD50 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 29390-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

hRad50, RAD50 homolog (S. cerevisiae)

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RAD50 Ready-To-Use IHC Kit. RAD50 primary antibody included.

产品信息

29390-1-PBS targets RAD50 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29949

Product name: Recombinant human RAD50 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 275-425 aa of NM_005732

Sequence: LDSRKKQMEKDNSELEEKMEKVFQGTDEQLNDLYHNHQRTVREKERKLVDCHRELEKLNKESRLLNQEKSELLVEQGRLQLQADRHQEHIRARDSLIQSLATQLELDGFERGPFSERQIKNFHKLVRERQEGEAKTANQLMNDFAEKETLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAD50 homolog (S. cerevisiae)
别名 hRad50, RAD50 homolog (S. cerevisiae)
计算分子量 154 kDa
观测分子量 154 kDa
GenBank蛋白编号NM_005732
基因名称 RAD50
Gene ID (NCBI) 10111
RRIDAB_2918289
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92878
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAD50 belongs to the ABC-ATPase protein family that concurs in forming the Nucleotide Binding Domain (NBD). ATP binding to Rad50 induces a conformational change that dramatically increases the Rad50 NBD affinity for itself, allowing for its dimerization (PMID: 37569756). The Mre11-Rad50 (MR) complex has key functions in the detection, signaling and repair of DNA breaks (PMID: 35501303).

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