验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, Raji cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy RAD17 Ready-To-Use IHC Kit. RAD17 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
13358-1-AP targets RAD17 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag4171 Product name: Recombinant human RAD17 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 421-670 aa of BC032304 Sequence: LVEPEEVVEMSHMPGDLFNLYLHQNYIDFFMEIDDIVRASEFLSFADILSGDWNTRSLLREYSTSIATRGVMHSNKARGYAHCQGGGSSFRPLHKPQWFLINKKYRENCLAAKALFPDFCLPALCRQTQLLPYLALLTIPMRNQAQISFIQDIGRLPLKRHFGRLKMEALTDREHGMIDPDSGDEAQLNGGHSAEESLGEPTQATVPETWSLPLSQNSASELPASQPQPFSAQGDMEENIIIEDYESDGT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAD17 homolog (S. pombe) |
| 别名 | HRAD17, R24L, RF-C/activator 1 homolog |
| 计算分子量 | 681 aa, 71 kDa |
| 观测分子量 | 71 kDa |
| GenBank蛋白编号 | BC032304 |
| 基因名称 | RAD17 |
| Gene ID (NCBI) | 5884 |
| RRID | AB_2269268 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75943 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for RAD17 antibody 13358-1-AP | Download protocol |
| IF protocol for RAD17 antibody 13358-1-AP | Download protocol |
| IHC protocol for RAD17 antibody 13358-1-AP | Download protocol |
| WB protocol for RAD17 antibody 13358-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogenesis The TIP60-ATM axis regulates replication fork stability in BRCA-deficient cells.
| ||
Biochem J Mutation of the RelA(p65) Thr505 phosphosite disrupts the DNA replication stress response leading to CHK1 inhibitor resistance | ||
