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  • Featured Product
  • KD/KO Validated

RAD17 Polyclonal antibody

RAD17 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13358-1-AP

Print datasheet

Synonyms

CCYC, HRAD17, R24L, RAD17, RAD17 homolog (S. pombe), RAD17SP, RAD24, RF C/activator 1 homolog

验证数据展示

  • WB analysis using 13358-1-AP

    WB analysis using 13358-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13358-1-AP (RAD17 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 13358-1-AP

    IHC staining of human breast cancer using 13358-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13358-1-AP (RAD17 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human breast cancer using 13358-1-AP

    IHC staining of human breast cancer using 13358-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13358-1-AP (RAD17 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 13358-1-AP

    IF Staining of HeLa using 13358-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13358-1-AP (RAD17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells, Raji cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13358-1-AP targets RAD17 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen RAD17 fusion protein Ag4171 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAD17 homolog (S. pombe)
Synonyms CCYC, HRAD17, R24L, RAD17, RAD17 homolog (S. pombe), RAD17SP, RAD24, RF C/activator 1 homolog
Calculated Molecular Weight 681 aa, 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC032304
Gene Symbol RAD17
Gene ID (NCBI) 5884
RRIDAB_2269268
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75943
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAD17 antibody 13358-1-APDownload protocol
IHC protocol for RAD17 antibody 13358-1-APDownload protocol
IF protocol for RAD17 antibody 13358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncogenesis

The TIP60-ATM axis regulates replication fork stability in BRCA-deficient cells.

Authors - Emily M Schleicher
  • KD Validated
View Article
mouseWB

Biochem J

Mutation of the RelA(p65) Thr505 phosphosite disrupts the DNA replication stress response leading to CHK1 inhibitor resistance

Authors - Jill E Hunter
View Article
mouseWB

Biochem J

Regulation of CHK1 inhibitor resistance by a c-Rel and USP1 dependent pathway

Authors - Jill E Hunter
View Article