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RAB9A-Specific Polyclonal antibody

RAB9A-Specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 18719-1-AP

Print datasheet

Synonyms

RAB9, RAB9A, RAB9A-Specific, Ras related protein Rab 9A

验证数据展示

  • WB analysis of HEK-293 using 18719-1-AP

    WB analysis of HEK-293 using 18719-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18719-1-AP (RAB9A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 18719-1-AP

    IHC staining of human gliomas using 18719-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18719-1-AP (RAB9A-Specific Antibody) at dilution of 1:50.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18719-1-AP targets RAB9A-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB9A, member RAS oncogene family
Synonyms RAB9, RAB9A, RAB9A-Specific, Ras related protein Rab 9A
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC017265
Gene Symbol RAB9A
Gene ID (NCBI) 9367
RRIDAB_2175604
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51151
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB9A, also named as RAB9, is a member of the Ras superfamily of small Rab GTPases. It is located in late endosomes. Rab9A interacts with its effector proteins p40 and TIP47 to promote the MPR (mannose 6-phosphate receptor)-associated lysosomal enzyme transport between late endosomes and the trans Golgi network. The antibody is specific to RAB9A.

实验方案

Product Specific Protocols
WB protocol for RAB9A-Specific antibody 18719-1-APDownload protocol
IHC protocol for RAB9A-Specific antibody 18719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols