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验证数据展示

  • WB analysis of HEK-293 using 18719-1-AP

    WB analysis of HEK-293 using 18719-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18719-1-AP (RAB9A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 18719-1-AP

    IHC staining of human gliomas using 18719-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18719-1-AP (RAB9A-Specific Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with RAB9A antibody included

RAB9A-Specific Polyclonal antibody

RAB9A-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18719-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

RAB9, RAB9A, RAB9A-Specific, Ras related protein Rab 9A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RAB9A Ready-To-Use IHC Kit. RAB9A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18719-1-AP targets RAB9A-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB9A, member RAS oncogene family
别名 RAB9, RAB9A, RAB9A-Specific, Ras related protein Rab 9A
计算分子量 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC017265
基因名称 RAB9A
Gene ID (NCBI) 9367
RRIDAB_2175604
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51151
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB9A, also named as RAB9, is a member of the Ras superfamily of small Rab GTPases. It is located in late endosomes. Rab9A interacts with its effector proteins p40 and TIP47 to promote the MPR (mannose 6-phosphate receptor)-associated lysosomal enzyme transport between late endosomes and the trans Golgi network. The antibody is specific to RAB9A.

实验方案

Product Specific Protocols
IHC protocol for RAB9A-Specific antibody 18719-1-APDownload protocol
WB protocol for RAB9A-Specific antibody 18719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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