验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, U-937 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 3 publications below |
产品信息
12283-1-AP targets RAB7B in WB, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, monkey |
| 免疫原 |
CatNo: Ag2936 Product name: Recombinant human RAB7B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-199 aa of BC017092 Sequence: MNPRKKVDLKLIIVGAIGVGKTSLLHQYVHKTFYEEYQTTLGASILSKIIILGDTTLKLQIWDTGGQERFRSMVSTFYKGSDGCILAFDVTDLESFEALDIWRGDVLAKIVPMEQSYPMVLLGNKIDLADRKVPQEVAQGWCREKDIPYFEVSAKNDINVVQAFEMLASRALSRYQSILENHLTESIKLSPDQSRSRCC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB7B, member RAS oncogene family |
| 别名 | RAB7, Ras related protein Rab 7b, Ras-related protein Rab-7b |
| 计算分子量 | 199 aa, 23 kDa |
| 观测分子量 | 27 kDa |
| GenBank蛋白编号 | BC017092 |
| 基因名称 | RAB7B |
| Gene ID (NCBI) | 338382 |
| RRID | AB_10640895 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96AH8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAB7B encodes a small GTPase that binds and hydrolyzes GTP and functions in vesicle trafficking. The protein, Ras-related protein Rab-7b, is localized to the Golgi apparatus, late endosome, lysosome, and phagocytic vesicle, placing it in compartments that support intracellular transport and sorting. Its expression is detected in skin, the proximal digestive tract, and the female reproductive system.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB7B antibody 12283-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
bioRxiv Legionella pneumophila exploits the endo-lysosomal network for phagosome biogenesis by co-opting SUMOylated Rab7 | ||
Biochem Biophys Res Commun Melanin-concentrating hormone receptor 1 is discarded by exosomes after internalization | ||
J Virol Nsp2 replicase-mediated viral uncoating in porcine alveolar macrophages contributes to the attenuation of PRRSV-2 live attenuated vaccine |
