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验证数据展示

  • WB analysis using 86951-1-RR

    WB analysis using 86951-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86951-1-RR (RAB5B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86951-1-RR

    WB analysis using 86951-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86951-1-RR (RAB5B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86951-1-RR

    WB analysis using 86951-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86951-1-RR (RAB5B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86951-1-RR

    WB analysis using 86951-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86951-1-RR (RAB5B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86951-1-RR

    WB analysis using 86951-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86951-1-RR (RAB5B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86951-1-RR

    WB analysis using 86951-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86951-1-RR (RAB5B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity of 86951-1-RR

    Affinity of 86951-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86951-1-RR against Human RAB5B were performed. The affinity constant is 0.668 nM.

RAB5B Recombinant monoclonal antibody

RAB5B Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86951-1-RR

产品说明书

CloneNo. 252038B1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

EC:3.6.5.2, Ras related protein Rab 5B, Ras-related protein Rab-5B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, NIH/3T3 cells, rat lung tissue, Recombinant protein, mouse lung tissue, HEK-293 cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86951-1-RR targets RAB5B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg5609

Product name: Recombinant human RAB5B protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 1-215 aa of NM_001252036.2

Sequence: MTSRSTARPNGQPQASKICQFKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQSVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNQETFARAKTWVKELQRQASPSIVIALAGNKADLANKRMVEYEEAQAYADDNSLLFMETSAKTAMNVNDLFLAIAKKLPKSEPQNLGGAAGRSRGVDLHEQSQQNKSQCCSN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB5B, member RAS oncogene family
别名 EC:3.6.5.2, Ras related protein Rab 5B, Ras-related protein Rab-5B
计算分子量24 kDa
观测分子量 24-27 kDa
GenBank蛋白编号NM_001252036.2
基因名称 RAB5B
Gene ID (NCBI) 5869
RRIDAB_3745229
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP61020-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB5B (Ras-related protein Rab-5B) belongs to the Rab family. Rab5 is specifically localized to the early compartment and regulates endocytosis (PMID:1516130, PMID:9087439). There are three isoforms of Rab5 namely, Rab5a, Rab5b, and Rab5c present in EE of mammalian cells (PMID:7789520). Recently, we have shown that Rab5a regulates fluid-phase endocytosis whereas receptor-mediated endocytosis is regulated by Rab5b in Leishmania (PMID:27226564).

实验方案

Product Specific Protocols
WB protocol for RAB5B antibody 86951-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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