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  • KD/KO Validated

RAB5A Polyclonal antibody

RAB5A Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11947-1-AP

Print datasheet

Synonyms

RAB5, RAB5A, Ras related protein Rab 5A



经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, mouse brain tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11947-1-AP targets RAB5A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Immunogen RAB5A fusion protein Ag2549 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB5A, member RAS oncogene family
Synonyms RAB5, RAB5A, Ras related protein Rab 5A
Calculated Molecular Weight 215 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC001267
Gene Symbol RAB5A
Gene ID (NCBI) 5868
RRIDAB_2269388
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20339
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAB5A antibody 11947-1-APDownload protocol
IHC protocol for RAB5A antibody 11947-1-APDownload protocol
IP protocol for RAB5A antibody 11947-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Genet

Identification of TMEM230 mutations in familial Parkinson's disease.

Authors - Han-Xiang Deng
humanWB,IP

Autophagy

Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.

Authors - Amy H Ponsford
mouseWB

Autophagy

RAB7 activity is required for the regulation of mitophagy in oocyte meiosis and oocyte quality control during ovarian aging.

Authors - Xin Jin
mouseWB,IHC

Diabetes

Atorvastatin Targets the Islet Mevalonate Pathway to Dysregulate mTOR Signaling and Reduce β-Cell Functional Mass.

Authors - Linyan Shen
  • KD Validated
ratWB, IF

Sci Signal

Semaphorin 3A activates the guanosine triphosphatase Rab5 to promote growth cone collapse and organize callosal axon projections.

Authors - Kong-Yan Wu
humanWB,IF,IP

Elife

Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.

Authors - Dawei Wang