验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31195-1-AP targets RAB44 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag35126 Product name: Recombinant human RAB44 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of NM_001257357 Sequence: METGQRTSRKVRKLGSNRRRQTREPADGEGAAVAPEPESWSSQAAAELQAFFQDCGAKERGFVTREDLAVAKFSFLGSKEESEMIFDWVDVERKGHLSLEEFSSGLKNIFGSSQSPHRLRRRKPLPSKRVSATTSFPALEEADAEEKEAF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB44, member RAS oncogene family |
| 别名 | RASL13, RASD3 |
| 计算分子量 | 111 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | NM_001257357 |
| 基因名称 | RAB44 |
| Gene ID (NCBI) | 401258 |
| RRID | AB_3669894 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q7Z6P3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rab44 is a member of the large Rab GTPase family, encoding an N-terminal EF-hand domain, a coiled-coil domain and a C-terminal Rab GTPase domain. It is highly expressed in immune-related cells such as mast cells, macrophages, osteoclasts and granulocyte lineage cells in the bone marrow. In mouse mast cells, Rab44 is expressed as two isoforms, the long and short forms with molecular masses of 180 kDa and 110 kDa (PMID: 33641252).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB44 antibody 31195-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
