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验证数据展示

  • WB analysis using 17827-1-AP

    WB analysis using 17827-1-AP

    Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 17827-1-AP (RAB3IL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17827-1-AP

    IF Staining of HeLa using 17827-1-AP

    Immunofluorescent analysis of Hela cells, using RAB3IL1 antibody 17827-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of mouse cerebellum using 17827-1-AP

    IHC staining of mouse cerebellum using 17827-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17827-1-AP (RAB3IL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 17827-1-AP

    IHC staining of mouse cerebellum using 17827-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17827-1-AP (RAB3IL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 17827-1-AP

    IHC staining of mouse cerebellum using 17827-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17827-1-AP (RAB3IL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RAB3IL1 Polyclonal antibody

RAB3IL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17827-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Rabin3-like 1, Rabin3 like 1, Rab3A-interacting-like protein 1, Rab-3A-interacting-like protein 1, Guanine nucleotide exchange factor for Rab-3A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected infetal human brain tissue, human testis tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17827-1-AP targets RAB3IL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 RAB3IL1 fusion protein Ag12204 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB3A interacting protein (rabin3)-like 1
别名 Rabin3-like 1, Rabin3 like 1, Rab3A-interacting-like protein 1, Rab-3A-interacting-like protein 1, Guanine nucleotide exchange factor for Rab-3A
计算分子量 382 aa, 43 kDa
观测分子量 42 kDa
GenBank蛋白编号BC022239
基因名称 RAB3IL1
Gene ID (NCBI) 5866
RRIDAB_2300814
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TBN0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAB3IL1 antibody 17827-1-APDownload protocol
IHC protocol for RAB3IL1 antibody 17827-1-APDownload protocol
IF protocol for RAB3IL1 antibody 17827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Small GTPases

Guanine nucleotide exchange factors activate Rab8a for Toll-like receptor signalling.

Authors - Samuel J Tong
View Article
mouseIF

Development

Identification of a heterogeneous and dynamic ciliome during embryonic development and cell differentiation

Authors - Kelsey H Elliott
View Article