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验证数据展示

  • WB analysis using 24599-1-AP

    WB analysis using 24599-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24599-1-AP (RAB3GAP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 24599-1-AP

    IHC staining of human brain using 24599-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24599-1-AP (RAB3GAP2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 24599-1-AP

    IHC staining of human brain using 24599-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24599-1-AP (RAB3GAP2 Antibody) at dilution of 1:50 (under 40x lens).

RAB3GAP2 Polyclonal antibody

RAB3GAP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24599-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

KIAA0839, p150, Rab3 GAP p150, Rab3 GAP regulatory subunit, RAB3 GAP150, RAB3GAP150, RAB3GAP2, RGAP iso

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, mouse brain tissue
Positive IHC detected inhuman brain tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24599-1-AP targets RAB3GAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 RAB3GAP2 fusion protein Ag20254 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB3 GTPase activating protein subunit 2 (non-catalytic)
别名 KIAA0839, p150, Rab3 GAP p150, Rab3 GAP regulatory subunit, RAB3 GAP150, RAB3GAP150, RAB3GAP2, RGAP iso
计算分子量 1393 aa, 156 kDa
观测分子量 150 kDa
GenBank蛋白编号BC146760
基因名称 RAB3GAP2
Gene ID (NCBI) 25782
RRIDAB_2879632
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H2M9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB3GAP2, also known as RAB3-GAP150, belongs to the Rab3-GAP regulatory subunit family. Rab3 GAP consists of two subunits: the catalytic subunit p130 and the noncatalytic subunit p150. Rab3 GAP is ubiquitously expressed and enriched in the synaptic soluble fraction of brain, consistent with it having a key role in neurodevelopment. RAB3-GAP150 forms the Rab3 GTPase-activating complex with RAB3-GAP130,where it constitutes the regulatory subunit, whereas the latter functions as the catalytic subunit. Defects in RAB3-GAP150 are the cause of Martsolf and Warburg Micro syndrome.

实验方案

Product Specific Protocols
WB protocol for RAB3GAP2 antibody 24599-1-APDownload protocol
IHC protocol for RAB3GAP2 antibody 24599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols