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验证数据展示

  • WB analysis of NCI-H1299 using 67118-1-Ig

    WB analysis of NCI-H1299 using 67118-1-Ig

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67118-1-Ig (RAB3D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67118-1-Ig

    IHC staining of human colon cancer using 67118-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67118-1-Ig (RAB3D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 67118-1-Ig

    IHC staining of human colon cancer using 67118-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67118-1-Ig (RAB3D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 67118-1-Ig

    IF Staining of A549 using 67118-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using RAB3D antibody (67118-1-Ig, Clone: 3C2F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

RAB3D Monoclonal antibody

RAB3D Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67118-1-Ig

产品说明书

CloneNo. 3C2F10

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, IHC, IF/ICC, ELISA

别名

D2 2, GOV, RAB16, RAB3D, RAD3D, Ras related protein Rab 3D

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNCI-H1299 cells, A549 cells, HT-29 cells, THP-1 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67118-1-Ig targets RAB3D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 RAB3D fusion protein Ag16784 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RAB3D, member RAS oncogene family
别名 D2 2, GOV, RAB16, RAB3D, RAD3D, Ras related protein Rab 3D
计算分子量 219 aa, 24 kDa
观测分子量 28 kDa
GenBank蛋白编号BC016471
基因名称 RAB3D
Gene ID (NCBI) 9545
RRIDAB_2882421
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95716
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAB3D antibody 67118-1-IgDownload protocol
IHC protocol for RAB3D antibody 67118-1-IgDownload protocol
IF protocol for RAB3D antibody 67118-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols