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验证数据展示

  • WB analysis using 12234-1-AP

    WB analysis using 12234-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12234-1-AP (RAB38 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 12234-1-AP

    IHC staining of human gliomas using 12234-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12234-1-AP (RAB38 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 12234-1-AP

    IHC staining of human gliomas using 12234-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12234-1-AP (RAB38 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 12234-1-AP

    IF Staining of A549 using 12234-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 12234-1-AP (RAB38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

RAB38 Polyclonal antibody

RAB38 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12234-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Melanoma antigen NY MEL 1, RAB 38, Ras related protein Rab 38, Ras-related protein Rab-38

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA375 cells, NIH/3T3 cells, HaCaT cells, MCF-10A cells
Positive IHC detected inhuman gliomas tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12234-1-AP targets RAB38 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 RAB38 fusion protein Ag2874 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB38, member RAS oncogene family
别名 Melanoma antigen NY MEL 1, RAB 38, Ras related protein Rab 38, Ras-related protein Rab-38
计算分子量 211 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC015808
基因名称 RAB38
Gene ID (NCBI) 23682
RRIDAB_2877839
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP57729
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB38 (also known as rrGTPbp or NY-MEL-1) is a member of the Rab small GTPase family, which regulates intracellular vesicle trafficking and membrane transport. RAB38 is specifically expressed in lysosome-related organelle (LRO)-containing tissues and cells, such as melanocytes and platelets, where it interacts with its paralogue RAB32 to regulate melanosome biogenesis and transport melanogenic enzymes (e.g., tyrosinase, TRP1, and TRP2) from early/recycling endosomes to maturing melanosomes.

实验方案

Product Specific Protocols
WB protocol for RAB38 antibody 12234-1-APDownload protocol
IHC protocol for RAB38 antibody 12234-1-APDownload protocol
IF protocol for RAB38 antibody 12234-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO Rep

Rab22A recruits BLOC-1 and BLOC-2 to promote the biogenesis of recycling endosomes.

Authors - Saurabh Shakya
View Article
mouseIF

JBMR Plus

Interspecies Single-Cell RNA-Seq Analysis Reveals the Novel Trajectory of Osteoclast Differentiation and Therapeutic Targets

Authors - Yasunori Omata
View Article