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验证数据展示

  • WB analysis of HeLa using 27349-1-AP (same clone as 27349-1-PBS)

    WB analysis of HeLa using 27349-1-AP (same clone as 27349-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 27349-1-AP (same clone as 27349-1-PBS)

    IHC staining of human stomach using 27349-1-AP (same clone as 27349-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27349-1-AP (RAB33B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 27349-1-AP (same clone as 27349-1-PBS)

    IHC staining of human stomach using 27349-1-AP (same clone as 27349-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27349-1-AP (RAB33B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

RAB33B Polyclonal antibody, PBS Only

RAB33B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27349-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

Ras-related protein Rab-33B, Ras related protein Rab 33B

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27349-1-PBS targets RAB33B in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag24181

Product name: Recombinant human RAB33B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 39-229 aa of BC036064

Sequence: IGDSNVGKTCLTYRFCAGRFPDHTEATIGVDFRERAVEIDGERIKIQLWDTAGQERFRKSMVQHYYRNVHAVVFVYDMTNMASFHSLPSWIEECKQHLLANDIPRILVGNKCDLRSAIQVPTDLAQKFADTHSMPLFETSAKNPNDNDHVEAIFMTLAHKLKSHKPLMLSQPPDNGIILKPEPKPAMTCWC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB33B, member RAS oncogene family
别名 Ras-related protein Rab-33B, Ras related protein Rab 33B
计算分子量 229 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC036064
基因名称 RAB33B
Gene ID (NCBI) 83452
RRIDAB_2880851
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H082
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAB33B is a small GTP-binding protein of the Rab GTPase family, whose members function in vesicle transport during protein secretion and endocytosis. Rab GTPases are active, membrane-associated proteins that recruit effector proteins in the GTP-bound state and inactive cytosolic proteins when in a GDP-bound state. RAB33B is ubiquitously expressed and has been implicated in Golgi to endoplasmic reticulum cycling of Golgi enzymes. In addition, RAB33B regulates Golgi homeostasis and coordinates intra-Golgi retrograde trafficking.

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