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验证数据展示

  • WB analysis of mouse brain using 11025-1-AP

    WB analysis of mouse brain using 11025-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11025-1-AP (RAB33A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 11025-1-AP

    IHC staining of human brain using 11025-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11025-1-AP (RAB33A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 11025-1-AP

    IHC staining of human brain using 11025-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11025-1-AP (RAB33A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-12 using 11025-1-AP

    IF Staining of PC-12 using 11025-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using RAB33A antibody (11025-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

RAB33A Polyclonal antibody

RAB33A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11025-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Small GTP-binding protein S10, Small GTP binding protein S10, Ras-related protein Rab-33A, Ras related protein Rab 33A, RABS10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue
Positive IHC detected inhuman brain tissue, human gliomas tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11025-1-AP targets RAB33A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性rat
免疫原 RAB33A fusion protein Ag1492 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB33A, member RAS oncogene family
别名 Small GTP-binding protein S10, Small GTP binding protein S10, Ras-related protein Rab-33A, Ras related protein Rab 33A, RABS10
计算分子量 27 kDa
观测分子量 27 kDa
GenBank蛋白编号BC009996
基因名称 RAB33A
Gene ID (NCBI) 9363
RRIDAB_2238178
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14088
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAB33A antibody 11025-1-APDownload protocol
IHC protocol for RAB33A antibody 11025-1-APDownload protocol
IF protocol for RAB33A antibody 11025-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB, IF

Biochem Biophys Res Commun

The small GTPase Rab33A participates in regulation of amylase release from parotid acinar cells.

Authors - Akane Imai
View Article