• Featured Product
  • KD/KO验证

RAB31-specific Polyclonal antibody

RAB31-specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16182-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (2)

Rab22B, RAB31, RAB31-specific, Ras related protein Rab 22B, Ras related protein Rab 31

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, K-562 cells
Positive IHC detected inhuman colon tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RAB31 Ready-To-Use IHC Kit. RAB31 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16182-1-AP targets RAB31-specific in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB31, member RAS oncogene family
别名 Rab22B, RAB31, RAB31-specific, Ras related protein Rab 22B, Ras related protein Rab 31
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号NM_006868
基因名称 RAB31
Gene ID (NCBI) 11031
RRIDAB_1851568
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13636
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB 31, also named as RAB22b, belongs to small GTP-binding proteins of the RAB family. Rab31 localizes in the trans-Golgi network (TGN) and endosomes. It is required for transport of mannose 6-phosphate receptors (MPRs) from the trans-Golgi network (TGN) to endosomes. This antibody can bind RAB31 specifically, and not react RAB22a.

实验方案

Product Specific Protocols
WB protocol for RAB31-specific antibody 16182-1-APDownload protocol
IHC protocol for RAB31-specific antibody 16182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Cell Res

RAB31 marks and controls an ESCRT-independent exosome pathway.

Authors - Denghui Wei
  • KD Validated
humanWB

Int J Biol Sci

Rab31 promotes the invasion and metastasis of cervical cancer cells by inhibiting MAPK6 degradation.

Authors - Yujie Huang
  • KD Validated
humanWB,IHC,CoIP

Front Oncol

RAB31 Targeted by MiR-30c-2-3p Regulates the GLI1 Signaling Pathway, Affecting Gastric Cancer Cell Proliferation and Apoptosis.

Authors - Chao-Tao Tang
  • KD Validated
humanWB

PLoS One

Human liver cell trafficking mutants: characterization and whole exome sequencing.

Authors - Fei Yuan
human,mouseWB,IHC,IF

Int J Biochem Cell Biol

Rab31 promotes activation of hepatic stellate cells by accelerating TGF-β receptor II complex endocytosis.

Authors - Chan Qiu
  • KD Validated
humanWB,IHC

Life Sci

Upregulation of Rab31 is associated with poor prognosis and promotes colorectal carcinoma proliferation via the mTOR/p70S6K/Cyclin D1 signalling pathway.

Authors - Li Yang
  • KD Validated
Loading...