验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, rat brain tissue, mouse kidney tissue, HeLa cells, mouse brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy RAB2B Ready-To-Use IHC Kit. RAB2B primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
11756-1-AP targets RAB2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | RAB2B fusion protein Ag2352 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB2B, member RAS oncogene family |
| 别名 | Ras-related protein Rab-2B, Ras related protein Rab 2B, EC:3.6.5.2 |
| 计算分子量 | 216 aa, 24 kDa |
| 观测分子量 | 24 kDa |
| GenBank蛋白编号 | BC020839 |
| 基因名称 | RAB2B |
| Gene ID (NCBI) | 84932 |
| RRID | AB_2176900 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WUD1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. This antibody can recognize both RAB2A and RAB2B.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB2B antibody 11756-1-AP | Download protocol |
| IHC protocol for RAB2B antibody 11756-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol A dual role of ERGIC-localized Rabs in TMED10-mediated unconventional protein secretion
| ||
Dis Markers Differential Plasma Proteins Identified via iTRAQ-Based Analysis Serve as Diagnostic Markers of Pancreatic Ductal Adenocarcinoma | ||
Dev Cell Setdb1 protects genome integrity in murine muscle stem cells to allow for regenerative myogenesis and inflammation |
