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RAB27A Polyclonal antibody
RAB27A Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (4)
Applications
IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HL-60 cells, Apoptosised HeLa cells, human liver tissue, K562, K-562 cells, L02 cells, Jurkat cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human malignant melanoma tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17817-1-AP targets RAB27A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | IHC, IP, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human |
Cited Reactivity | human, rat, Chicken, pig, canine |
Immunogen | RAB27A fusion protein Ag12136 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | RAB27A, member RAS oncogene family |
Synonyms | GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A |
Calculated Molecular Weight | 221 aa, 25 kDa |
Observed Molecular Weight | 25-27 kDa |
GenBank Accession Number | BC107680 |
Gene Symbol | RAB27A |
Gene ID (NCBI) | 5873 |
RRID | AB_2176728 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P51159 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for RAB27A antibody 17817-1-AP | Download protocol |
IHC protocol for RAB27A antibody 17817-1-AP | Download protocol |
IP protocol for RAB27A antibody 17817-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Immunol Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity. | ||
J Allergy Clin Immunol A RAB27A 5' untranslated region structural variant associated with late-onset hemophagocytic lymphohistiocytosis and normal pigmentation. | ||
Nat Commun Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.
| ||
Blood Extracellular vesicles released by CD40/IL-4 stimulated chronic lymphocytic leukemia cells confer altered functional properties to CD4+ T cells. |