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验证数据展示

  • WB analysis of MCF-7 using 84776-2-RR

    WB analysis of MCF-7 using 84776-2-RR

    WB result of RAB21 antibody (84776-2-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB21 transfected MCF-7 cells.

  • WB analysis using 84776-2-RR

    WB analysis using 84776-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84776-2-RR (RAB21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 84776-2-RR

    IHC staining of human colon using 84776-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84776-2-RR (RAB21 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 84776-2-RR

    IHC staining of mouse testis using 84776-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84776-2-RR (RAB21 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 84776-2-RR

    IHC staining of mouse testis using 84776-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84776-2-RR (RAB21 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 84776-2-RR

    IHC staining of human colon using 84776-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84776-2-RR (RAB21 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 84776-2-RR

    IF Staining of HeLa using 84776-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB21 antibody (84776-2-RR, Clone: 242117C4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84776-2-RR

    Affinity and Kinetic Characterization of 84776-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84776-2-RR against Human RAB21 were performed. The affinity constant is 0.388 nM.

  • Featured Product
  • KD/KO验证

RAB21 Recombinant monoclonal antibody

RAB21 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84776-2-RR

产品说明书

CloneNo. 242117C4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

242117C4, KIAA0118, Ras related protein Rab 21

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, HepG2 cells, LNCaP cells, MDA-MB-231 cells
Positive IHC detected inhuman colon tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84776-2-RR targets RAB21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag35291

Product name: Recombinant human RAB21 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 115-225 aa of NM_014999

Sequence: KELRKMLGNEICLCIVGNKIDLEKERHVSIQEAESYAESVGAKHYHTSAKQNKGIEELFLDLCKRMIETAQVDERAKGNGSSQPGTARRGVQIIDDEPQAQTSGGGCCSSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB21, member RAS oncogene family
别名 242117C4, KIAA0118, Ras related protein Rab 21
计算分子量24 kDa
观测分子量24 kDa
GenBank蛋白编号NM_014999
基因名称 RAB21
Gene ID (NCBI) 23011
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UL25
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Small GTPase Rab21 is a ubiquitously expressed and has recently been shown to associate with the α tails of several integrins via the shared conserved membrane proximal sequence, thus regulating cell adhesion and migration via controlling the endo/exocytic trafficking of most integrin heterodimers (PMID: 18804435). Rab21 is required for the pruning of the immature neurites during the multipolar-to-bipolar transition (PMID: 36683567). Knock down of Rab21 impairs integrin-mediated cell adhesion and motility, whereas its overexpression stimulates cell migration and cancer cell adhesion to collagen and human bone (PMID: 16754960).

实验方案

Product Specific Protocols
IF protocol for RAB21 antibody 84776-2-RRDownload protocol
IHC protocol for RAB21 antibody 84776-2-RRDownload protocol
WB protocol for RAB21 antibody 84776-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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