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验证数据展示

  • WB analysis using 29110-1-AP (same clone as 29110-1-PBS)

    WB analysis using 29110-1-AP (same clone as 29110-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29110-1-AP (RAB20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29110-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 29110-1-AP (same clone as 29110-1-PBS)

    IHC staining of human liver cancer using 29110-1-AP (same clone as 29110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29110-1-AP (RAB20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29110-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 29110-1-AP (same clone as 29110-1-PBS)

    IHC staining of human liver cancer using 29110-1-AP (same clone as 29110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29110-1-AP (RAB20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29110-1-PBS in a different storage buffer formulation.

RAB20 Polyclonal antibody, PBS Only

RAB20 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29110-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29110-1-PBS targets RAB20 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30459

Product name: Recombinant human RAB20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 115-234 aa of BC026025

Sequence: VDLTEEGALAGQEKEECSPNMDAGDRVSPRAPKQVQLEDAVALYKKILKYKMLDEQDVPAAEQMCFETSAKTGYNVDLLFETLFDLVVPMILQQRAERPSHTVDISSHKPPKRTRSGCCA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB20, member RAS oncogene family
别名
计算分子量 26 kDa
观测分子量 29 kDa
GenBank蛋白编号BC026025
基因名称 RAB20
Gene ID (NCBI) 55647
RRIDAB_3086099
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NX57
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAB20 is a member of RAS oncogene family, it has no close relationship to any other members of RAB subfamily which consists of small GTP-binding proteins involved in the regulation of intracellullar vesicular transport. Firstly found highly expressed on the apical side of kidney tubuel epithelial cells, RAB20 was speculated to be involved in apical endocytosi and recylcing processes. So far, its function is not well known, however, RAB20 is recently found higher expressed in pancreatic adenocarcinomas than normal pancreas, which is proved by WB and IHC analyses, suggesting its promising role in diagnosis of pancreatic carcinogenesis. Catalog#11616-1-AP is a rabbit polyclonal antibody raised against full-length of human RAB20.

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