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验证数据展示

  • WB analysis using 83687-4-RR (same clone as 83687-4-PBS)

    WB analysis using 83687-4-RR (same clone as 83687-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83687-4-RR (RAB1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83687-4-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83687-4-RR (same clone as 83687-4-PBS)

    FC experiment of U-251 using 83687-4-RR (same clone as 83687-4-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug RAB1B Recombinant antibody (83687-4-RR, Clone:240635D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83687-4-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83687-4-RR (same clone as 83687-4-PBS)

    IHC staining of human ovary cancer using 83687-4-RR (same clone as 83687-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83687-4-RR (RAB1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83687-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAB1B antibody included
  • IHC staining of human ovary cancer using 83687-4-RR (same clone as 83687-4-PBS)

    IHC staining of human ovary cancer using 83687-4-RR (same clone as 83687-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83687-4-RR (RAB1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83687-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAB1B antibody included
  • FC experiment of HeLa using 83687-4-RR (same clone as 83687-4-PBS)

    FC experiment of HeLa using 83687-4-RR (same clone as 83687-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAB1B Recombinant antibody (83687-4-RR, Clone:240635D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83687-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 83687-4-RR (same clone as 83687-4-PBS)

    IHC staining of mouse heart using 83687-4-RR (same clone as 83687-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83687-4-RR (RAB1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83687-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAB1B antibody included
  • IHC staining of mouse heart using 83687-4-RR (same clone as 83687-4-PBS)

    IHC staining of mouse heart using 83687-4-RR (same clone as 83687-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83687-4-RR (RAB1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83687-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAB1B antibody included
  • IF Staining of NIH/3T3 using 83687-4-RR (same clone as 83687-4-PBS)

    IF Staining of NIH/3T3 using 83687-4-RR (same clone as 83687-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RAB1B antibody (83687-4-RR, Clone: 240635D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83687-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83687-4-RR

    Affinity and Kinetic Characterization of 83687-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83687-4-RR against Human RAB1B were performed. The affinity constant is below 1 pM.

RAB1B Recombinant monoclonal antibody, PBS Only

RAB1B Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83687-4-PBS

产品说明书

CloneNo. 240635D9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

240635D9, EC:3.6.5.2, Ras related protein Rab 1B, Ras-related protein Rab-1B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RAB1B Ready-To-Use IHC Kit. RAB1B primary antibody included.

产品信息

83687-4-PBS targets RAB1B in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12197

Product name: Recombinant human RAB1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-201 aa of BC071169

Sequence: MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVGNKSDLTTKKVVDNTTAKEFADSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASGGERPNLKIDSTPVKPAGGGCC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB1B, member RAS oncogene family
别名 240635D9, EC:3.6.5.2, Ras related protein Rab 1B, Ras-related protein Rab-1B
计算分子量 201 aa, 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC071169
基因名称 RAB1B
Gene ID (NCBI) 81876
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9H0U4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ras-related protein Rab-1B (RAB1B) belongs to the small GTPase superfamily and Rab family. Rab1b, a GTPase regulating secretory transport, was recently associated with targeting proteins to LDs in a Drosophila RNAi screen. Rab1b recruits lipid-synthesizing enzymes, facilitating Lipid droplets (LDs) growth. The small GTPase Rab1b interacts with diacylglycerol acyltransferase 2 to facilitate its targeting to the surface of lipid droplets. (PMID: 38809969). Rab1b, an extensively studied and established master regulator of ER-to-Golgi transport. The mechanism of Rab1b's function is to promote ER to LD surface targeting of those triglyceride-synthesizing enzymes that can associate with the unique lipid monolayer comprising the LD surface (PMID: 38809969). Rab1b is a regulatory protein involved in both COPI and COPII transport (PMID: 17429068).

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