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- KD/KO Validated
RAB1B Polyclonal antibody
RAB1B Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
IF/ICC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A375 cells, HeLa cells, HAP1, A431 cells, NIH/3T3 cells |
Positive IHC detected in | human heart tissue, mouse heart tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, NIH/3T3 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17824-1-AP targets RAB1B in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC, CoIP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | RAB1B fusion protein Ag12197 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | RAB1B, member RAS oncogene family |
Synonyms | RAB1B, Ras related protein Rab 1B |
Calculated Molecular Weight | 201 aa, 22 kDa |
Observed Molecular Weight | 22-25 kDa |
GenBank Accession Number | BC071169 |
Gene Symbol | RAB1B |
Gene ID (NCBI) | 81876 |
RRID | AB_2237881 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9H0U4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for RAB1B antibody 17824-1-AP | Download protocol |
IHC protocol for RAB1B antibody 17824-1-AP | Download protocol |
IF protocol for RAB1B antibody 17824-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens. | ||
Aging (Albany NY) Overexpression of Rab1B and MMP9 predicts poor survival and good response to chemotherapy in patients with colorectal cancer. | ||
Front Pharmacol Fuyuan Xingnao Decoction Promotes Angiogenesis Through the Rab1/AT1R Pathway in Diabetes Mellitus Complicated With Cerebral Infarction. | ||
Oncol Rep Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer. |