验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HepG2 cells, human brain tissue, mouse kidney tissue, Raji cells, rat brain tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15662-1-AP targets RAB14 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 |
CatNo: Ag8092 Product name: Recombinant human RAB14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-215 aa of BC006081 Sequence: MATAPYNYSYIFKYIIIGDMGVGKSCLLHQFTEKKFMADCPHTIGVEFGTRIIEVSGQKIKLQIWDTAGQERFRAVTRSYYRGAAGALMVYDITRRSTYNHLSSWLTDARNLTNPNTVIILIGNKADLEAQRDVTYEEAKQFAEENGLLFLEASAKTGENVEDAFLEAAKKIYQNIQDGSLDLNAAESGVQHKPSAPQGGRLTSEPQPQREGCGC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB14, member RAS oncogene family |
| 别名 | EC:3.6.5.2, FBP, RAB 14, Ras related protein Rab 14, Ras-related protein Rab-14 |
| 计算分子量 | 215 aa, 24 kDa |
| 观测分子量 | 24 kDa |
| GenBank蛋白编号 | BC006081 |
| 基因名称 | RAB14 |
| Gene ID (NCBI) | 51552 |
| RRID | AB_2173630 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P61106 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAB14, the final member of the Rab11 subfamily, participates in the regulation of cell secretion, ensuring the timely release of necessary substances outside the cell. RAB14 is ubiquitously expressed and localizes to the Golgi/TGN and at early endosomes (PMID: 16962593, 22595670). RAB14 contains 215 amino acids, with a molecular weight of approximately 24.6 kDa
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for RAB14 antibody 15662-1-AP | Download protocol |
| WB protocol for RAB14 antibody 15662-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Interruption of post-Golgi STING trafficking activates tonic interferon signaling
| ||
Acta Pharm Sin B A rationally designed CD19 monoclonal antibody-triptolide conjugate for the treatment of systemic lupus erythematosus | ||
J Exp Clin Cancer Res Circular RNA hsa_circ_0000326 acts as a miR-338-3p sponge to facilitate lung adenocarcinoma progression. | ||
FASEB J Rab14-Mediated Lipophagy Disrupts Lipid Homeostasis in Granulosa Cells and Impairs Female Fertility. | ||
BMC Cancer MiR-214-3p targets Ras-related protein 14 (RAB14) to inhibit cellular migration and invasion in esophageal Cancer cells | ||
J Virol The Small GTPase Rab14 Regulates the Trafficking of Ceramide from Endoplasmic Reticulum to Golgi Apparatus and Facilitates Classical Swine Fever Virus Assembly
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