验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, HUVEC cells, A549 cells, LNCaP cells, U-251 cells, HSC-T6 cells, RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
68079-1-Ig targets RAB12 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag31627 Product name: Recombinant human RAB12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 105-244 aa of NM_001025300 Sequence: NSITSAYYRSAKGIILVYDITKKETFDDLPKWMKMIDKYASEDAELLLVGNKLDCETDREITRQQGEKFAQQITGMRFCEASAKDNFNVDEIFLKLVDDILKKMPLDILRNELSNSILSLQPEPEIPPELPPPRPHVRCC 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | RAB12, member RAS oncogene family |
| 别名 | FLJ45927, RAB12, Ras related protein Rab 12 |
| 计算分子量 | 27KD |
| 观测分子量 | 27-30 kDa |
| GenBank蛋白编号 | NM_001025300 |
| 基因名称 | RAB12 |
| Gene ID (NCBI) | 201475 |
| RRID | AB_2918816 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q6IQ22 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB12 antibody 68079-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
