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验证数据展示

  • WB analysis using 29927-1-AP

    WB analysis using 29927-1-AP

    Human brain tissue was subjected to SDS PAGE followed by western blot with 29927-1-AP (RAB12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 29927-1-AP

    IP experiment of mouse brain using 29927-1-AP

    IP result of anti-RAB12 (IP:29927-1-AP, 4ug; Detection:29927-1-AP 1:1500) with mouse brain tissue lysate 2100 ug.

RAB12 Polyclonal antibody

RAB12 Polyclonal Antibody for WB, IP, ELISA
Cat No. 29927-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

Ras-related protein Rab-12, Ras related protein Rab 12

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman brain tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29927-1-AP targets RAB12 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31627

Product name: Recombinant human RAB12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 105-244 aa of NM_001025300

Sequence: NSITSAYYRSAKGIILVYDITKKETFDDLPKWMKMIDKYASEDAELLLVGNKLDCETDREITRQQGEKFAQQITGMRFCEASAKDNFNVDEIFLKLVDDILKKMPLDILRNELSNSILSLQPEPEIPPELPPPRPHVRCC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB12, member RAS oncogene family
别名 Ras-related protein Rab-12, Ras related protein Rab 12
计算分子量27KD
观测分子量 27 kDa
GenBank蛋白编号NM_001025300
基因名称 RAB12
Gene ID (NCBI) 201475
RRIDAB_3669700
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6IQ22
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab12, a previously uncharacterized Rab isoform, is mainly localized at TfR-positive recycling endosomes and is specifically involved in TfR degradation. Rab12 is a regulator of LRRK2 and its activation by damaged lysosomes.

实验方案

Product Specific Protocols
IP protocol for RAB12 antibody 29927-1-APDownload protocol
WB protocol for RAB12 antibody 29927-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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