验证数据展示

WB analysis using 20229-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 20229-1-AP
WB result of RAB11A-Specific antibody (20229-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB11A-Specific transfected HeLa cells.
IHC staining of human small intestine using 20229-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 10x lens).
Ready-to-use IHC kits available with RAB11A/B antibody included
IF Staining of HEK-293 using 20229-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RAB11A-Specific antibody (20229-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IHC staining of human small intestine using 20229-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 40x lens).
Ready-to-use IHC kits available with RAB11A/B antibody included
IHC staining of mouse brain using 20229-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with RAB11A/B antibody included
IHC staining of mouse brain using 20229-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with RAB11A/B antibody included
WB analysis of MCF-7 using 20229-1-AP
WB result of RAB11A antibody (20229-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB11A transfected MCF-7 cells.
IP experiment of SH-SY5Y using 20229-1-AP
IP result of anti-RAB11A (IP:20229-1-AP, 4ug; Detection:20229-1-AP 1:1000) with SH-SY5Y cells lysate 1840 ug.

Featured Product
KD/KO Validated

RAB11A Polyclonal antibody

RAB11A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20229-1-AP

产品说明书

引用文献(33)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

RAB11A-Specific, Rab 11, RAB11, RAB11A/B, EC:3.6.5.2

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (33)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HEK-293T cells, HeLa cells, human brain tissue, MCF-7 cells, mouse colon tissue, PC-12 cells, rat brain tissue, SH-SY5Y cells, mouse lung tissue
Positive IP detected in	SH-SY5Y cells
Positive IHC detected in	human small intestine tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Planning an IHC experiment? We recommend our IHCeasy RAB11A/B Ready-To-Use IHC Kit. RAB11A/B primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 6 publications below
WB	See 20 publications below
IHC	See 1 publications below
IF	See 18 publications below
IP	See 1 publications below

产品信息

20229-1-AP targets RAB11A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, canine
免疫原	Peptide 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	RAB11A, member RAS oncogene family
别名	RAB11A-Specific, Rab 11, RAB11, RAB11A/B, EC:3.6.5.2
计算分子量	24 kDa
观测分子量	24 kDa
GenBank蛋白编号	NM_004663
基因名称	RAB11A
Gene ID (NCBI)	8766
RRID	AB_10666202
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P62491
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939). The antibody is specific to RAB11A, and has no cross reaction to RAB11B.

实验方案

Product Specific Protocols
IF protocol for RAB11A antibody 20229-1-AP	Download protocol
IHC protocol for RAB11A antibody 20229-1-AP	Download protocol
IP protocol for RAB11A antibody 20229-1-AP	Download protocol
WB protocol for RAB11A antibody 20229-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
		Nature Interactions between TTYH2 and APOE facilitate endosomal lipid transfer Authors - Anastasiia Sukalskaia View Article
human	WB	Immunity Targeting intracellular oncoproteins with dimeric IgA promotes expulsion from the cytoplasm and immune-mediated control of epithelial cancers Authors - Subir Biswas View Article
human	WB,IF	J Extracell Vesicles RAB22A sorts epithelial growth factor receptor (EGFR) from early endosomes to recycling endosomes for microvesicles release Authors - Yujie Lin View Article
mouse	IF	Autophagy PLD1 promotes Spindle Assembly and migration through regulating autophagy in mouse oocyte meiosis Authors - Jiaqi Zhang View Article
human	WB	Nat Commun Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane Authors - Tong Chen KD Validated View Article
mouse	WB,IF	Adv Sci (Weinh) CCDC41 Drives Oocyte Meiotic Progression by Promoting Rab11a/Rab7-Positive Vesicle Fusion with Target Membranes. Authors - Ying Tian View Article

验证数据展示

WB analysis using 20229-1-AP

WB analysis of HeLa using 20229-1-AP

IHC staining of human small intestine using 20229-1-AP

IF Staining of HEK-293 using 20229-1-AP

IHC staining of human small intestine using 20229-1-AP

IHC staining of mouse brain using 20229-1-AP

IHC staining of mouse brain using 20229-1-AP

WB analysis of MCF-7 using 20229-1-AP

IP experiment of SH-SY5Y using 20229-1-AP

RAB11A Polyclonal antibody

RAB11A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Interactions between TTYH2 and APOE facilitate endosomal lipid transfer

Targeting intracellular oncoproteins with dimeric IgA promotes expulsion from the cytoplasm and immune-mediated control of epithelial cancers

RAB22A sorts epithelial growth factor receptor (EGFR) from early endosomes to recycling endosomes for microvesicles release

PLD1 promotes Spindle Assembly and migration through regulating autophagy in mouse oocyte meiosis

Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane

CCDC41 Drives Oocyte Meiotic Progression by Promoting Rab11a/Rab7-Positive Vesicle Fusion with Target Membranes.

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